小鼠血管内皮生长因子 ELISA定量试剂盒(VEGF)

小鼠血管内皮生长因子 (VEGF) ELISA定量试剂盒

英文名称：Mouse VEGF ELISA Kit    货号：KTE7016-1

产品定位

• 业务领域：细胞分析（核心产品）
• 适用类别：蛋白类 > ELISA应用
• 检测类型：夹心法 ELISA
• 检测指标：VEGF（血管内皮生长因子）

适用样本

细胞培养上清、血清、血浆及其他生物液体（小鼠来源）。

试剂盒组成

组件	说明
小鼠源 VEGF‑A 抗体预包被微孔板	96孔/48孔，已固定捕获抗体
小鼠源 VEGF‑A 蛋白标准品	提供系列稀释浓度，用于绘制标准曲线
小鼠源 VEGF‑A 检测抗体	生物素标记，配合 Streptavidin‑HRP 检测
EliKine™ Streptavidin‑HRP	高灵敏度酶标二抗
标准品稀释液	用于标准品的准确稀释
实验缓冲液	提供均一的反应环境
HRP 底物	TMB 颜色显色剂
反应终止液	停止酶反应，固定终点颜色
洗涤缓冲液（含 Tween-20）	高效去除非特异性结合
封板膜	防止板面蒸发与污染

性能指标

• 检测范围：15.63 pg/mL – 1000 pg/mL
• 灵敏度（LOD）：8 pg/mL
• 特异性：已验证对 VEGF‑A 同源蛋白无显著交叉反应，背景干扰低

使用说明要点

• 所有试剂仅限本批号使用，勿与其他批次混合。
• 使用前请在室温（≈25 °C）平衡30 分钟。
• 实验操作建议使用一次性吸头，防止交叉污染。
• 未使用的板条应立即返回包装，密封后置于 4 °C 保存。
• 混匀对结果影响显著，推荐使用低速振荡器或每10 分钟手动轻摇。
• 样品与标准建议设置双孔或三孔（复孔）以增强数据可靠性。

存储与运输

• 未开封：2 °C – 8 °C 保存。
• 启用后：请遵循说明书中各组分的具体保存条件。
• 运输方式：配合蓝色冰袋冷链运输，确保全程低温。

安全与合规

本产品为研究用试剂，不适用于人类或临床诊断。使用时请遵守当地法规，避免侵犯专利或许可证。厂商不承担因违规使用导致的任何责任。

技术背景

血管内皮生长因子（VEGF），亦称血管通透性因子（VPF），是调控血管生成、通透性以及内皮细胞增殖的关键分子。VEGF 以二硫键形成同源二聚体糖蛋白，分子量约 34–45 kDa。小鼠 VEGF‑A 与人类 VEGF‑165 在功能上高度保守，广泛参与肿瘤血管生成、糖尿病性视网膜病变等病理过程。对 VEGF‑A 的准确定量能够帮助科研团队解析血管生成机制、评估药物干预效果以及筛选潜在治疗靶点。

产品规格及价格

规格	包装	单价（人民币）	货期
KTE7016-1	48 T	1,598.00	现货（次日发货）
KTE7016-1	96 T	2,558.00	现货（次日发货）

检索信息

基因 ID：22339  |  蛋白 ID：Q00731  |  别名：VEGFA, VPF, VEGF‑A, VEGF165  |  常用关键词：血管内皮生长因子 ELISA 试剂盒, Mouse VEGF ELISA Kit, KTE7016

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Vascular Endothelial Growth Factor (VEGF) ELISA Quantitative Kit

English Name: Mouse VEGF ELISA Kit    Catalog Number: KTE7016-1

Product Positioning

• Business Area: Cell Analysis (Core Product)
• Suitable Category: Protein > ELISA Application
• Assay Type: Sandwich ELISA
• Detection Target: VEGF (Vascular Endothelial Growth Factor)

Suitable Samples

Cell culture supernatants, serum, plasma, and other biological fluids (mouse origin).

Kit Components

Component	Description
Pre-coated Microplate with Mouse VEGF-A Antibody	96-well/48-well, pre-coated with capture antibody
Mouse VEGF-A Protein Standard	Provided with serial dilution concentrations for standard curve generation
Mouse VEGF-A Detection Antibody	Biotin-labeled, for use with Streptavidin-HRP detection
EliKine™ Streptavidin-HRP	High-sensitivity enzyme-labeled secondary antibody
Standard Diluent	For accurate dilution of standards
Assay Buffer	Provides a uniform reaction environment
HRP Substrate	TMB color development reagent
Stop Solution	Stops the enzyme reaction and fixes the endpoint color
Wash Buffer (contains Tween-20)	Effectively removes non-specific binding
Plate Sealer	Prevents evaporation and contamination

Performance Characteristics

• Detection Range: 15.63 pg/mL – 1000 pg/mL
• Sensitivity (LOD): 8 pg/mL
• Specificity: Validated for no significant cross-reactivity with VEGF-A homologous proteins; low background interference

Key Usage Instructions

• All reagents are for use with this batch only; do not mix with reagents from other batches.
• Equilibrate all reagents to room temperature (≈25 °C) for 30 minutes before use.
• Use disposable pipette tips to prevent cross-contamination.
• Unused strips should be returned to the foil pouch immediately, sealed, and stored at 4 °C.
• Thorough mixing significantly impacts results; use a low-speed shaker or gently shake manually every 10 minutes.
• It is recommended to set up duplicates or triplicates for samples and standards to enhance data reliability.

Storage and Shipping

• Unopened: Store at 2 °C – 8 °C.
• After Opening: Please follow the specific storage conditions for each component as indicated in the manual.
• Shipping Method: Shipped with blue ice packs via cold chain to ensure low temperature throughout transit.

Safety and Compliance

This product is intended for research use only, not for human use or clinical diagnosis. Please comply with local regulations and avoid infringement of patents or licenses. The manufacturer assumes no liability for any consequences resulting from improper use.

Technical Background

Vascular Endothelial Growth Factor (VEGF), also known as Vascular Permeability Factor (VPF), is a key molecule regulating angiogenesis, vascular permeability, and endothelial cell proliferation. VEGF forms homodimeric glycoproteins via disulfide bonds, with a molecular weight of approximately 34–45 kDa. Mouse VEGF-A is highly conserved in function with human VEGF-165 and is widely involved in pathological processes such as tumor angiogenesis and diabetic retinopathy. Accurate quantification of VEGF-A can assist research teams in elucidating angiogenesis mechanisms, evaluating drug intervention effects, and screening potential therapeutic targets.

Specifications and Pricing

Specification	Packaging	Price (RMB)	Availability
KTE7016-1	48 T	1,598.00	In Stock (Ships Next Day)
KTE7016-1	96 T	2,558.00	In Stock (Ships Next Day)

Reference Information

Gene ID: 22339  |  Protein ID: Q00731  |  Aliases: VEGFA, VPF, VEGF-A, VEGF165  |  Common Keywords: Vascular Endothelial Growth Factor ELISA Kit, Mouse VEGF ELISA Kit, KTE7016

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakoyin Company for specific details.