二肽基多肽酶IV酶联免疫试剂盒(人) DPPIV/CD26

Human DPPIV/CD26 ELISA Kit (人二肽基多肽酶IV酶联免疫试剂盒)

货号: KTE3085-3

产品分类: 核心产品 - 细胞分析

反应性: 人

检测方法: 双抗体夹心法ELISA

样本类型: 细胞培养上清、血清、血浆

产品概述

EliKine™ Human DPPIV/CD26 ELISA Kit是一种高度特异性和灵敏的定量检测试剂盒，专门设计用于精准检测人样本中的二肽基多肽酶IV（DPPIV/CD26）蛋白水平。该试剂盒采用经典的夹心法ELISA原理，结合高亲和力抗体对，确保检测结果的可靠性和准确性，适用于生命科学研究、药物开发及疾病生物标志物分析等领域。

主要特点与优势

• 高灵敏度: 最低检测限达15.63 pg/mL，可精准检测低丰度样本。
• 优异特异性: 经严格验证，对目标蛋白类似物无明显交叉反应或背景干扰。
• 宽检测范围: 15.63 pg/mL - 1,000 pg/mL，适应不同浓度样本的检测需求。
• 操作便捷: 预包被微孔板设计，优化缓冲液体系，简化实验流程。
• 批次稳定性: 严格质量控制，确保不同批次间结果的一致性。

试剂盒组分

• Human DPPIV 预包被微孔板
• Human DPPIV 标准品（冻干）
• 10× 检测缓冲液
• 100× Human DPPIV 检测抗体（生物素标记）
• 100× 链霉亲和素-HRP 偶联物
• TMB 底物溶液
• 终止液
• 20× 洗涤缓冲液
• 板盖

背景信息

二肽基多肽酶IV（DPPIV/CD26）是一种广泛表达于细胞表面的多功能跨膜糖蛋白，兼具丝氨酸外肽酶活性。它在免疫调节、信号转导、葡萄糖代谢及细胞凋亡等生理过程中扮演关键角色。DPPIV通过降解肠促胰岛素（如GLP-1）参与血糖调控，并与肝脏、肾脏等器官的纤维化进程相关。此外，DPPIV在肿瘤生物学中作为重要的生物标志物，其表达水平在多种癌症中呈现动态变化，且被发现可作为MERS-CoV的细胞表面受体，凸显其在病原体感染机制研究中的价值。

技术参数

参数	详情
检测范围	15.63 - 1,000 pg/mL
灵敏度	15.63 pg/mL
样本体积	建议参考说明书，通常为50-100 μL
孵育时间	总实验时间约3-4小时（含准备与孵育）
检测波长	450 nm（参考波长可选630 nm）

保存与运输

保存条件: 未开封试剂盒请避光保存于2-8℃，有效期24个月；启用后请按说明书要求保存各组分。

运输条件: 冰袋（蓝冰）运输，确保试剂活性。

注意事项

• 请勿混合使用不同批号或过期试剂。
• 使用前所有试剂需室温平衡30分钟。
• 实验过程中请使用一次性吸头，避免交叉污染。
• 未使用的板条请立即密封，4℃保存。
• 充分混匀试剂对结果准确性至关重要，推荐使用低速振荡器或定期手动摇匀。
• 为提高数据可靠性，建议对样品和标准品进行复孔检测。

订购信息

规格	货号	价格（元）	货期
48 T	KTE3085-3	1598.0	请咨询
96 T	KTE3085-3	2558.0	请咨询

警告: 本产品仅用于科学研究，不适用于人类或临床诊断。使用者应确保应用不违反相关专利或许可规定，厂家不承担因使用本产品可能导致的专利侵权或其他违规责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human DPPIV/CD26 ELISA Kit

Cat. No.: KTE3085-3

Product Category: Core Products - Cell Analysis

Reactivity: Human

Detection Method: Sandwich ELISA

Sample Type: Cell culture supernatant, serum, plasma

Product Overview

The EliKine™ Human DPPIV/CD26 ELISA Kit is a highly specific and sensitive quantitative assay kit, specifically designed for the precise detection of Dipeptidyl Peptidase IV (DPPIV/CD26) protein levels in human samples. This kit utilizes the classic sandwich ELISA principle, combined with high-affinity antibody pairs, to ensure reliable and accurate detection results. It is suitable for life science research, drug development, disease biomarker analysis, and other fields.

Key Features & Advantages

• High Sensitivity: Minimum detection limit of 15.63 pg/mL, enabling precise detection of low-abundance samples.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity with target protein analogs or background interference.
• Wide Detection Range: 15.63 pg/mL - 1,000 pg/mL, adaptable to samples with varying concentration requirements.
• User-Friendly Operation: Pre-coated microplate design and optimized buffer system simplify the experimental procedure.
• Batch-to-Batch Consistency: Strict quality control ensures consistent results across different batches.

Kit Components

• Human DPPIV Pre-coated Microplate
• Human DPPIV Standard (Lyophilized)
• 10× Assay Buffer
• 100× Human DPPIV Detection Antibody (Biotin-labeled)
• 100× Streptavidin-HRP Conjugate
• TMB Substrate Solution
• Stop Solution
• 20× Wash Buffer
• Plate Sealer

Background Information

Dipeptidyl Peptidase IV (DPPIV/CD26) is a multifunctional transmembrane glycoprotein widely expressed on the cell surface, possessing serine exopeptidase activity. It plays key roles in immune regulation, signal transduction, glucose metabolism, and apoptosis. DPPIV is involved in blood glucose regulation by degrading incretin hormones (such as GLP-1) and is associated with fibrosis processes in organs like the liver and kidneys. Furthermore, DPPIV serves as an important biomarker in tumor biology, with its expression levels dynamically changing in various cancers. It has also been identified as a cell surface receptor for the Middle East Respiratory Syndrome Coronavirus (MERS-CoV), highlighting its value in studying pathogen infection mechanisms.

Technical Parameters

Parameter	Details
Detection Range	15.63 - 1,000 pg/mL
Sensitivity	15.63 pg/mL
Sample Volume	Refer to the manual; typically 50-100 μL
Incubation Time	Total assay time approximately 3-4 hours (including preparation and incubation)
Detection Wavelength	450 nm (reference wavelength optional at 630 nm)

Storage & Shipping

Storage Conditions: Unopened kit should be stored protected from light at 2-8°C; shelf life is 24 months. After opening, store components as instructed.

Shipping Conditions: Shipped with ice packs (blue ice) to ensure reagent activity.

Precautions

• Do not mix reagents from different batches or use expired reagents.
• Allow all reagents to reach room temperature for 30 minutes before use.
• Use disposable pipette tips during the assay to avoid cross-contamination.
• Immediately seal unused strips and store at 4°C.
• Thorough mixing of reagents is crucial for accurate results; use a low-speed shaker or mix manually periodically.
• For improved data reliability, duplicate wells for samples and standards are recommended.

Ordering Information

Size	Cat. No.	Price (RMB)	Availability
48 T	KTE3085-3	1598.0	Please Inquire
96 T	KTE3085-3	2558.0	Please Inquire

Warning: This product is for research use only. Not for use in human or clinical diagnosis. The user should ensure that its application does not infringe relevant patents or licensing regulations. The manufacturer assumes no liability for patent infringement or other violations resulting from the use of this product.

Note: The above information is subject to change due to product optimization and upgrades. Please contact Yakeyin Company for specific details.