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- 文献和实验
- 技术资料
- 免疫原:
CD117
- 亚型:
recombinant human IgG1
- 形态:
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件:
避光,2-8℃
- 克隆性:
REA791
- 标记物:
Biotin
- 适应物种:
mouse
- 保质期:
24个月
- 供应商:
Miltenyi Biotec
- 宿主:
human cell line
- 应用范围:
Flow cytometry
- 浓度:
1:50
- 抗体英文名:
CD117 Antibody, anti-mouse, REAfinity™
- 抗体名:
CD117 Antibody, anti-mouse, REAfinity™
- 规格:
150 µg in 1 mL
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文献和实验suspension. 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6) Add a pre-titrated
of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
2000™ or stock 3.4µM MPG (3µl/ml of media described in (Morris et al. 2004)) (note 3).Pulldown (1), Antibody specific Forty-eight hours following the biotin linked sense or antisense siRNA transfection the cultures are collected and a chromatin
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