3-磷酸甘油醛脱氢酶试剂盒，用于样本中GAPDH活性检测，微

3-磷酸甘油醛脱氢酶（GAPDH）活性检测试剂盒（微量法）

产品编号：KTB1001-S

产品类型：细胞代谢检测试剂盒

产品概述

CheKine™ 3-磷酸甘油醛脱氢酶（GAPDH）活性检测试剂盒（微量法）是一款基于比色法原理，专业用于检测动植物组织、细胞、细菌、培养液及其他液体样本中GAPDH酶活性的高灵敏度试剂盒。GAPDH作为糖酵解途径中的关键酶，其活性检测在代谢研究、疾病机制探索及药物筛选等领域具有重要科研价值。

检测原理

本试剂盒基于酶偶联反应原理。在反应体系中，3-磷酸甘油酸激酶催化底物生成1,3-二磷酸甘油酸，随后GAPDH逆向催化该产物与NADH反应，生成NAD⁺。通过测定340 nm波长下NADH的吸光度下降速率，即可准确计算出GAPDH的酶活性水平。

试剂盒组成

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

特点与优势

• 经验证适用于多种样本类型，兼容性强
• 操作流程优化，简便高效，适合高通量筛选
• 灵敏度高，重复性好，结果稳定可靠

适用样本类型

适用于动植物组织、细胞提取物、细菌裂解液、培养上清及其他生物液体样本。

保存与运输

保存条件：-20℃避光保存，有效期6个月。

运输方式：蓝冰冰袋运输，保障试剂稳定性。

注意事项

• 推荐使用新鲜样本。若需保存，请于-80℃冻存，数月内稳定。
• 解冻过程需严格控制温度与时间，室温解冻应在4小时内完成检测。
• 实验前请确保所有试剂及设备处于适宜温度，以保证反应体系稳定性。

规格与价格

警告：本产品仅限于科研用途，不适用于人类或临床诊断。使用者应确保其应用不违反相关专利或许可协议。对于因使用本产品可能导致的专利侵权或其他违规行为，本公司不承担任何责任。

别名：3-磷酸甘油醛脱氢酶（GAPDH）；GAPDH Activity Assay Kit；Micro GAPDH Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit (Micro Method)

Catalog Number: KTB1001-S

Product Type: Cell Metabolism Assay Kit

Overview

CheKine™ Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit (Micro Method) is a highly sensitive colorimetric-based kit designed for the detection of GAPDH enzyme activity in animal and plant tissues, cells, bacteria, culture supernatants, and other liquid samples. As a key enzyme in the glycolytic pathway, the detection of GAPDH activity holds significant research value in metabolism studies, disease mechanism exploration, and drug screening.

Principle

This kit is based on an enzyme-coupled reaction principle. In the reaction system, phosphoglycerate kinase catalyzes the substrate to generate 1,3-bisphosphoglycerate, which is subsequently reversely catalyzed by GAPDH to react with NADH, producing NAD⁺. By measuring the rate of decrease in NADH absorbance at 340 nm, the GAPDH enzyme activity level can be accurately calculated.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

Features & Advantages

• Validated for a variety of sample types with strong compatibility
• Optimized, simple, and efficient procedure suitable for high-throughput screening
• High sensitivity, good reproducibility, and reliable results

Applicable Sample Types

Suitable for animal and plant tissues, cellular extracts, bacterial lysates, culture supernatants, and other biological liquid samples.

Storage & Shipping

Storage Conditions: Store protected from light at -20°C; stable for 6 months.

Shipping Method: Shipped with blue ice packs to ensure reagent stability.

Notes

• Fresh samples are recommended. If storage is necessary, freeze at -80°C; stable for several months.
• Thawing process must be strictly controlled in terms of temperature and time; samples thawed at room temperature should be assayed within 4 hours.
• Ensure all reagents and equipment are at appropriate temperatures prior to the experiment to maintain reaction system stability.

Specifications & Pricing

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. Users should ensure that their application does not infringe relevant patents or licensing agreements. The company assumes no liability for any patent infringement or other violations resulting from the use of this product.

Also Known As: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH); GAPDH Activity Assay Kit; Micro GAPDH Assay Kit

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please contact Acokine Inc.