Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-CAPG2 (bs-7721R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD
- ￥1180 - 2800
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- bs-7721R
- 2025年10月25日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7721R |
| 英文名称 | CAPG2 Rabbit pAb |
| 中文名称 | 染色体相关蛋白2抗体 |
| 英文别名 | NCAPG2; Chromosome associated protein G2; LUZP5; CAP G2; Leucine zipper protein 5; Non SMC condensin II complex subunit G2; Condensin 2 complex subunit G2; NCAPG 2; Non SMC condensin II complex, subunit G2; CNDG2_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat, human tonsil, human tonsil, human tonsil, human tonsil |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CAPG2/NCAPG2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 131 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell Division > Chromatid Cohesion Epigenetics and Nuclear Signaling > Cell cycle > Chromosome Structure > Chromatid Cohesion |
| 亚基 | Component of the condensin-2 complex, which contains the SMC2 and SMC4 heterodimer, and 3 non SMC subunits that probably regulate the complex: NCAPH2, NCAPD3 and NCAPG2. |
| 亚细胞定位 | Nucleus. |
| 相似性 | Contains 1 HEAT repeat. |
| 功能 | Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | NCAPG2 is 1 of 3 non-SMC subunits that define condensin II. Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4, but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that defines condensin II. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2ug/Test} |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CAPG2 antibody (bs-7721R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CAPG2 antibody (bs-7721R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human A431 cell lysates
Lane 4: Human MCF-7 cell lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human U251 cell lysates
Lane 7: Human Jurkat cell lysates
Primary: Anti-CAPG2 (bs-7721R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 131 kDa
Lane 1: Mouse Thymus tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human A431 cell lysates
Lane 4: Human MCF-7 cell lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human U251 cell lysates
Lane 7: Human Jurkat cell lysates
Primary: Anti-CAPG2 (bs-7721R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 131 kDa
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CAPG2) Polyclonal Antibody, Unconjugated (bs-7721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 8.44 }}] {Wang Wei. et al. Circular RNA circ0001955 promotes cervical cancer tumorigenesis and metastasis via the miR-188-3p/NCAPG2 axis. J TRANSL MED. 2023 Dec;21(1):1-18} {IHC,WB} {Human,Mouse}
[IF={{ 64.8 }}] {El Yakoubi Warif. et al. Condensin dysfunction is a reproductive isolating barrier in mice. NATURE. 2023 Nov;:1-8} {IF,WB} {Mouse}
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CAPG2 Rabbit pAb(bs-7721R)-50ul/100ul/200ul
￥1180 - 2800
