- ￥5800
- Proteintech
- 66737-1-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA
- Mouse
- human
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IL-1B protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA
- 靶点：
IL-1 beta
- 抗体英文名：
IL-1 beta Monoclonal antibody, PBS Only (Detector)
- 抗体名：
IL-1 beta Monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
66737-1-PBS targets IL-1 beta as part of a matched antibody pair:
MP80001-6: 82696-8-PBS capture and 66737-1-PBS detection (validated in Sandwich ELISA)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag26030 Product name: Recombinant human IL-1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 117-269 aa of BC008678 Sequence: APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 1, beta |
| 别名 | 2A1B4, Catabolin, IL 1, IL 1 beta, IL1 beta |
| 计算分子量 | 269 aa, 31 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC008678 |
| 基因名称 | IL1B |
| Gene ID (NCBI) | 3553 |
| ENSEMBL Gene ID | ENSG00000125538 |
| RRID | AB_2882087 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P01584 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.
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文献和实验panzerking 我想用IL-1beta 刺激HepG2细胞 用NF-kB-luc报告基因来检测nfkb的激活 想请教刺激前是否要用无血清的培养液饥饿细胞 或者是将IL-1溶解在PBS里 用PBS来刺激细胞 细胞是否不能培养在5%的血清培养液中呢 请战友指点 谢谢 螺旋 转染NF-kB-luc报告基因24小时以后,换成无血清的培养基继续培养2-10个小时,需要看你的细胞
隆抗体，脱脂奶粉用于单克隆抗体，这样可得到较高的信噪比。预染的蛋白质Marker，可用于监测转膜的效率。 样品制备 原始样品可为细胞、组织、培养上清、免疫沉淀或亲和纯化的蛋白，以下为 定性检测目的蛋白时细胞样品的处理方法，其余的样品制备方法参阅相关文献。 1.培养细胞或药物处理。 2.弃培养基，用1X PBS漂洗细胞2次，去尽残留培养基。 3.加入1X SDS样品缓冲液(6-well plate,100μl /w或 75 cm2 plate
Size‐Exclusion Chromatography with On‐Line Light Scattering
.6.6 Chromatograms of E. coli SCF. CHO SCF contains >30% carbohydrate and thus the three‐detector method was used. 100 µl of 3.8 mg/ml of E. coli SCF was injected into a Superdex 200 column (Pharmacia) with PBS as eluent at a flow rate of 0.5 ml/min. The solid line
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