重组人CD40配体，His-标签（无动物源）（CD40L）

重组人CD40配体，His-标签（无动物源）[PRP1920]

产品概述

• 应用领域：细胞培养研究，聚焦于免疫调控与细胞信号传导机制
• 生物活性：ED₅₀值<5 ng/mL（基于诱导人PBMCs分泌IL-8能力验证）
• 种属特异性：人源重组蛋白（UniProt ID: P29965）
• 生产体系：大肠杆菌表达系统，确保无动物源成分

核心优势

• 超低内毒素：<0.1 EU/μg（LAL法验证）
• 高纯度保障：SDS-PAGE检测纯度>95%
• 严格质控：功能性活性验证批次放行
• 即用型冻干：预充无菌PBS缓冲液（pH8.0）

技术参数

• 分子量：16 kDa（非还原条件）
• 结构特征：Met113-Leu261活性片段
• 蛋白形式：可溶性His标签融合蛋白
• 别称：TNFSF5/CD154/TRAP/Gp39

规格与订购

• 5 μg | ¥588 | 现货
• 20 μg | ¥1,498 | 现货
• 100 μg | ¥3,289 | 需询货期
• 定制规格：支持1 mg级大包装定制

使用指南

• 复溶建议：使用配套缓冲液复溶（浓度≥100 μg/mL）
• 保存方案：
  • 冻干粉：-20°C保存12个月
  • 工作液：添加稳定剂后-80°C保存6个月
• 实验提示：首次使用前需离心处理冻干粉

生物功能

作为TNF超家族关键成员，CD40L通过结合APC细胞表面CD40受体，调控B细胞活化、抗体类别转换及促炎因子分泌，在适应性免疫应答中发挥核心作用。

警告声明：本产品仅限于科研用途，不可用于临床诊断或治疗应用。使用前请充分阅读相关专利文献。 ```

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human CD40 Ligand, His-Tag (Animal-Free) [PRP1920]

Product Overview

• Application: Cell culture research, focusing on immune regulation and cell signaling mechanisms
• Biological Activity: ED₅₀ <5 ng/mL (based on IL-8 secretion induction in human PBMCs)
• Species Specificity: Human recombinant protein (UniProt ID: P29965)
• Production System: E. coli expression system, ensuring animal-free components

Core Advantages

• Ultra-Low Endotoxin: <0.1 EU/μg (verified by LAL assay)
• High Purity: >95% purity by SDS-PAGE
• Strict Quality Control: Functional activity verified for batch release
• Ready-to-Use Lyophilized Form: Pre-filled with sterile PBS buffer (pH 8.0)

Technical Parameters

• Molecular Weight: 16 kDa (non-reducing conditions)
• Structural Feature: Met113-Leu261 active fragment
• Protein Form: Soluble His-tagged fusion protein
• Aliases: TNFSF5/CD154/TRAP/Gp39

Specifications & Ordering

• 5 μg | ¥588 | In Stock
• 20 μg | ¥1,498 | In Stock
• 100 μg | ¥3,289 | Lead Time Required
• Custom Specifications: Supports 1 mg bulk packaging customization

Usage Guidelines

• Reconstitution Recommendation: Reconstitute with provided buffer (concentration ≥100 μg/mL)
• Storage Solution:
  • Lyophilized Powder: Store at -20°C for 12 months
  • Working Solution: Store at -80°C for 6 months after adding stabilizer
• Experimental Tip: Centrifuge lyophilized powder before first use

Biological Function

As a key member of the TNF superfamily, CD40L regulates B cell activation, antibody class switching, and pro-inflammatory cytokine secretion by binding to the CD40 receptor on APC cells, playing a central role in adaptive immune responses.

Warning Statement: This product is for research use only and not for clinical diagnosis or therapeutic applications. Please read relevant patent literature thoroughly before use. ```

Note: The above information may change due to product optimization and upgrades. For specific information, please consult Abbkine.