EliKine™ 小鼠肿瘤坏死因子相关凋亡诱导配体ELISA

EliKine™ 小鼠肿瘤坏死因子相关凋亡诱导配体ELISA定量试剂盒

产品编号：KTE3034 | 分子靶标：TRAIL/TNFSF10

核心规格：

• 48T规格：¥1598 | 96T规格：¥2558（具体货期请咨询客服）
• 检测模式：双抗体夹心法ELISA | 样本兼容性：血清/血浆/细胞培养上清

技术优势：

• 超高灵敏度：15.63 pg/mL检测下限
• 宽动态范围：15.63-1000 pg/mL线性覆盖
• 严格验证：无种属交叉反应，批间差＜10%

完整试剂盒组成

• 预包被微孔板 | 冻干标准品组
• 10×检测缓冲液 | 生物素化检测抗体
• 链霉亲和素-HRP复合物 | TMB显色系统

实验质控要点：

• 推荐三复孔设计 | 低速振荡混匀
• 全程低温运输（2-8℃蓝冰）
• 开封后组分按说明书分装保存

检测原理：

采用双表位捕获技术，通过预包被抗体和生物素化检测抗体的协同作用，结合HRP-TMB显色系统，实现对小鼠TRAIL蛋白的特异性定量检测。通过标准曲线可实现pg级精准定量，适用于细胞凋亡机制研究及炎症因子监测。

⚠ 科研专用：本产品不适用于临床诊断

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse TNF-Related Apoptosis-Inducing Ligand ELISA Quantitation Kit

Cat.No: KTE3034 | Target: TRAIL/TNFSF10

Key Specifications:

• 48T: ¥1598 | 96T: ¥2558 (Contact for lead time)
• Assay Type: Sandwich ELISA | Matrix Compatibility: Serum/Plasma/Cell Culture Supernatant

Technical Advantages:

• Ultra-high Sensitivity: 15.63 pg/mL detection limit
• Broad Dynamic Range: 15.63-1000 pg/mL linear detection
• Rigorous Validation: No cross-species reactivity, inter-assay CV <10%

Complete Kit Components

• Pre-coated Microplate | Lyophilized Standard Set
• 10× Assay Buffer | Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate | TMB Substrate System

Quality Control Guidelines:

• Recommended triplicate well design | Low-speed orbital mixing
• Cryogenic transport (2-8°C blue ice)
• Post-opening aliquot storage per manual

Detection Principle:

Utilizes dual-epitope capture technology with pre-coated capture antibody and biotinylated detection antibody, combined with HRP-TMB chromogenic system for specific quantitation of murine TRAIL. Achieves pg-level precision through standard curve analysis, suitable for apoptosis mechanism studies and inflammatory cytokine monitoring.

⚠ For research use only: Not intended for clinical diagnostics

Note: Specifications subject to change due to product optimization. Contact Yakornin Inc. for latest information.