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- 详细信息
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human c-Kit Protein (Site-specific APC-conjugated, His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
25 Tests/100 Tests
| 规格: | 25 Tests | 产品价格: | ¥2980.0 |
|---|---|---|---|
| 规格: | 100 Tests | 产品价格: | ¥9980.0 |
重组人c-Kit蛋白(产品说明)
蛋白名称:Human c-Kit Protein (Site-specific APC-conjugated, ECD, His Tag)
蛋白构建:A DNA sequence encoding the human Kit (P10721-2) (Met 1-Thr 516) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 664 nm).
表达宿主:HEK293 Cells
蛋白活性:1.Flow cytometric analysis of anti-c-Kit CAR expression. 5e5 of anti-c-Kit CAR-293 cells were stained with 100 μL of 1:125 dilution of APC-conjugated human c-Kit Protein (Site-specific APC-conjugated, His Tag, Cat. No. 11996-H86H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested). 2.Binding activity of APC-conjugated c-Kit protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human c-Kit Protein (Site-specific APC-conjugated, His Tag, Cat. No. 11996-H86H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gln 26
蛋白分子量:The protein has a predicted molecular mass of 58.6 kDa.
蛋白NP号:P10721-2
蛋白氨基酸序列:Met1-Thr516
蛋白名称:Human c-Kit Protein (Site-specific APC-conjugated, ECD, His Tag)
蛋白构建:A DNA sequence encoding the human Kit (P10721-2) (Met 1-Thr 516) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 664 nm).
表达宿主:HEK293 Cells
蛋白活性:1.Flow cytometric analysis of anti-c-Kit CAR expression. 5e5 of anti-c-Kit CAR-293 cells were stained with 100 μL of 1:125 dilution of APC-conjugated human c-Kit Protein (Site-specific APC-conjugated, His Tag, Cat. No. 11996-H86H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested). 2.Binding activity of APC-conjugated c-Kit protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human c-Kit Protein (Site-specific APC-conjugated, His Tag, Cat. No. 11996-H86H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gln 26
蛋白分子量:The protein has a predicted molecular mass of 58.6 kDa.
蛋白NP号:P10721-2
蛋白氨基酸序列:Met1-Thr516
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重组人c-Kit蛋白
¥2980 - 9980





