Fluorescein-dT Phosphoramidite

Oligodeoxyribonucleotides Synthesis: Phosphoramidite Approach

The development of deoxyribonucleoside phosphoramidite derivatives for the synthesis of oligodeoxyribonucleotides was first described by Beaucage and Caruthers ( 1 ) in 1981. The conceptual basis of this methodology emerged as a modification

Oligoribonucleotide Synthesis: The Silyl-Phosphoramidite Method

In 1968, one of us began a quest to develop a general method for the synthesis of RNA sequences. The major problems associated with the chemical assembly of ribonucleotide chains were already well established. RNA sequences are very sensitive

Labeling of Oligonucleotides with Fluorescein

Oligonucleotides are a convenient alternative to conventional, cloned DNA probes since they can be rapidly synthesized in large quantities relatively cheaply. They can be made to very specific regions of the genome, without reference