L929细胞

L929/L929细胞系/L929细胞株/L929小鼠成纤维细胞

Cell line name NCTC clone 929

Synonyms NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); Clone 929; Earles's cells; Earle's L cells

Accession CVCL_0462

Resource Identification Initiative To cite this cell line use: NCTC clone 929 (RRID:CVCL_0462)

Comments Group: Space-flown cell line (cellonaut).

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).

Doubling time: 28 hours (Note=From cell counting), 32 hours (Note=From absorbance) (DOI=10.5897/IJBMBR2013.0154); ~25 hours (CLS=400260); ~21-24 hours (DSMZ=ACC-2).

Omics: Deep quantitative proteome analysis.

Anecdotal: First established continuous cell line.

Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (16) than that of NIST (17,18) due to a change in the marker motif (personal communication of Almeida, Jamie L.).

Derived from site: In situ; Connective tissue; UBERON=UBERON_0002384.

Cell type: Fibroblast; CL=CL_0000057.

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: C3H/An.

Hierarchy Children:

CVCL_DD99 (1R [Mouse L cells]) CVCL_3984 (A-9) CVCL_4114 (B82)

CVCL_8887 (L Cells (TK+, HBS Ag+)) CVCL_IW04 (L-929 D) CVCL_IW05 (L-929 E)

CVCL_IW06 (L-929 F) CVCL_IW07 (L-929 HH) CVCL_4535 (L-M)

CVCL_U990 (L.P3) CVCL_0D72 (L4.5) CVCL_AR58 (L929/A)

CVCL_E2Z7 (L929/CD275) CVCL_C5ES (L929/CD83) CVCL_4237 (L929/R)

CVCL_4238 (L929S) CVCL_4Z39 (LA-9) CVCL_B950 (LAG)

CVCL_WY44 (LMCAT) CVCL_3742 (McCoy) CVCL_4255 (NCTC 2071)

CVCL_A0ZP (NCTC 2168) CVCL_C8QV (Wnt-LIGHT)

Sex of cell Male

Age at sampling 100D

Category Spontaneously immortalized cell line

STR profile Source(s): DSMZ=ACC-2; PubMed=23430347; PubMed=31220119; Ubigene=YC-C091

 

Markers:

Mouse STR 1-1 10

Mouse STR 1-2 17

Mouse STR 2-1 9

Mouse STR 3-2 13,14

Mouse STR 4-2 20.3

Mouse STR 5-5 14

Mouse STR 6-4 16,17 (DSMZ=ACC-2)

17,18 (PubMed=23430347; PubMed=31220119; Ubigene=YC-C091)

Mouse STR 6-7 12

Mouse STR 7-1 25,26,27

Mouse STR 8-1 16

Mouse STR 9-2 15

Mouse STR 11-2 15,16

Mouse STR 12-1 16

Mouse STR 13-1 17

Mouse STR 15-3 24.3,25.3,26.3

Mouse STR 17-2 15

Mouse STR 18-3 16

Mouse STR 19-2 12

Mouse STR X-1 26,27

 

Run an STR similarity search on this cell line

PubMed=18105872; DOI=10.1093/jnci/9.3.229

Sanford K.K., Earle W.R., Hobbs G.L.

The growth in vitro of single isolated tissue cells.

J. Natl. Cancer Inst. 9:229-246(1948)

 

PubMed=13293658

Sanford K.K., Hobbs G.L., Earle W.R.

The tumor-producing capacity of strain L mouse cells after 10 years in vitro.

Cancer Res. 16:162-166(1956)

 

PubMed=13431913; DOI=10.3181/00379727-94-23044

Foley G.E., Handler A.H.

Differentiation of normal and neoplastic cells maintained in tissue culture by implantation into normal hamsters.

Proc. Soc. Exp. Biol. Med. 94:661-664(1957)

 

PubMed=13587550; DOI=10.1083/jcb.4.5.567; PMCID=PMC2224560

Westfall B.B., Peppers E.V., Evans V.J., Sanford K.K., Hawkins N.M., Fioramonti M.C., Kerr H.A., Hobbs G.L., Earle W.R.

The arginase and rhodanese activities of certain cell strains after long cultivation in vitro.

J. Biophys. Biochem. Cytol. 4:567-570(1958)

 

PubMed=14185313; DOI=10.1126/science.146.3641.241

Cell Culture Collection Committee

Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.

Science 146:241-243(1964)

 

DOI=10.1007/BF02618370

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.

The animal cell culture collection.

In Vitro 5:1-16(1970)

 

PubMed=4328993; DOI=10.1016/0014-4827(71)90412-5

Takaoka T., Katsuta H.

Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media.

Exp. Cell Res. 67:295-304(1971)

 

PubMed=4560675

Tsuboi K., Takaoka T., Katsuta H.

Comparative analyses of strain L-929 and its substrain L-P3 grown in a protein- and lipid-free synthetic medium.

Jpn. J. Exp. Med. 42:361-376(1972)

 

PubMed=4471172; DOI=10.1007/BF02615345

Ham R.G.

Nutritional requirements of primary cultures. A neglected problem of modern biology.

In Vitro 10:119-129(1974)

 

PubMed=6298990; DOI=10.1016/0378-1135(82)90056-6

McPhee D.A., Parsonson I.M., Della-Porta A.J.

Comparative studies on the growth of Australian bluetongue virus serotypes in continuous cell lines and embryonated chicken eggs.

Vet. Microbiol. 7:401-410(1982)

 

DOI=10.4051/ibc.2010.2.4.0014

Yoshino K., Saijo K., Noro C., Nakamura Y.

Development of a simple method to determine the mouse strain from which cultured cell lines originated.

Interdisciplinary Bio Central 2:1-9(2010)