3T3-L1细胞

3T3-L1/3T3-L1细胞系/3T3-L1细胞株/3T3-L1小鼠胚胎成纤维细胞

Cell line name 3T3-L1

Synonyms 3T3 L1; 3T3L1; 3T3-L1 ad; NIH-3T3-L1; NIH3T3-L1

Accession CVCL_0123

Resource Identification Initiative To cite this cell line use: 3T3-L1 (RRID:CVCL_0123)

Comments Doubling time: ~2.2 days (Note=Lot 12142004), 31 hours (Note=Lot 07032008), ~28 hours (Note=Lot 07232012), ~54 hours (Note=Lot 02202015), ~39 hours (Note=Lot 08012016), ~28 hours (Note=Lot 02212018) (JCRB=IFO50416); ~43 hours (Note=Lot 11272012), ~41 hours (Note=Lot 02132012), ~28 hours (Note=Lot 08222011), ~23 hours (Note=Lot 02152011), ~26 hours (Note=Lot 01142010), ~31 hours (Note=Lots 01282009 and 08222016), ~1 day (Note=Lot 01252008), ~1.5 days (Note=Lot 02092007), ~33 hours (Note=Lots 06122006 and 05252015), ~40 hours (Note=Lot 12162004), 60-70 hours (Note=Lot 06082004), ~3 days (Note=Lot 10302002), ~37 hours (Note=Lot 032499), ~30 hours (Note=Lot 06012015), 20-64 hours (Note=Lot 04212017), ~27 hours (Note=Lot 10082020) (JCRB=JCRB9014).

Miscellaneous: STR profile from personal communication of Rodriguez-Tarduchy Segovia, Gemma.

Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.

Cell type: Fibroblast; CL=CL_0000057.

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: Swiss albino.

Hierarchy Parent: CVCL_0120 (3T3-Swiss albino)

Children:

CVCL_D4DA (3T3-L1 CAR SNRK KO) CVCL_QZ17 (3T3-L1 EGFP-ATA2) CVCL_0A20 (3T3-L1 MBX)

CVCL_0122 (3T3F442A) CVCL_D6A9 (HyCyte 3T3-L1 KO-mAckr3) CVCL_E1IK (HyCyte 3T3-L1 KO-mFasn)

Sex of cell Male

Age at sampling Embryo

Category Spontaneously immortalized cell line

STR profile Source(s): Direct_author_submission

 

Markers:

Mouse STR 1-1 10,15

Mouse STR 1-2 13

Mouse STR 2-1 9

Mouse STR 3-2 14

Mouse STR 4-2 19.3

Mouse STR 5-5 13,15

Mouse STR 6-4 15.3

Mouse STR 6-7 12,15

Mouse STR 7-1 25.2,29

Mouse STR 8-1 15,16

Mouse STR 11-2 15

Mouse STR 12-1 19

Mouse STR 13-1 15

Mouse STR 15-3 20.3

Mouse STR 17-2 12,15

Mouse STR 18-3 17,21

Mouse STR 19-2 12

Mouse STR X-1 26

 

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Publications

DOI=10.1016/0092-8674(74)90126-3

Green H., Kehinde O.

Sublines of mouse 3T3 cells that accumulate lipid.

Cell 1:113-116(1974)

 

PubMed=4426090; DOI=10.1016/0092-8674(74)90116-0

Green H., Meuth M.

An established pre-adipose cell line and its differentiation in culture.

Cell 3:127-133(1974)

 

PubMed=165899; DOI=10.1016/0092-8674(75)90087-2

Green H., Kehinde O.

An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion.

Cell 5:19-27(1975)

 

Patent=US4003789

Green H.

Triglyceride-accumulating clonal cell line.

Patent number US4003789, 18-Jan-1977

 

PubMed=6698114; DOI=10.1016/0014-4827(84)90361-6

Levine J.F., Stockdale F.E.

3T3-L1 adipocytes promote the growth of mammary epithelium.

Exp. Cell Res. 151:112-122(1984)

 

PubMed=28176856; DOI=10.1038/srep42104; PMCID=PMC5296734

Kassotis C.D., Masse L., Kim S., Schlezinger J.J., Webster T.F., Stapleton H.M.

Characterization of adipogenic chemicals in three different cell culture systems: implications for reproducibility based on cell source and handling.

Sci. Rep. 7:42104-42104(2017)