C2C12细胞

C2C12/C2C12细胞系/C2C12细胞株/C2C12小鼠成肌细胞

Cell line name C2C12

Synonyms C2c12; C2-C12; C12

Accession CVCL_0188

Resource Identification Initiative To cite this cell line use: C2C12 (RRID:CVCL_0188)

Comments Part of: ENCODE project mouse cell lines.

Characteristics: Capable, upon starvation, of differentiating into contractile myotubes that express muscle-specific proteins.

Doubling time: 19.6 +- 0.4 hours (Note=At 22th passage), 18.4 +- 0.2 (Note=At 38th passage) (PubMed=22020321); ~20 hours (DSMZ=ACC-565).

Omics: Cell surface proteome.

Omics: Deep proteome analysis.

Omics: H3K27me3 ChIP-seq epigenome analysis.

Omics: H3K36me3 ChIP-seq epigenome analysis.

Omics: H3K4me3 ChIP-seq epigenome analysis.

Omics: H3K79me2 ChIP-seq epigenome analysis.

Omics: H3K79me3 ChIP-seq epigenome analysis.

Omics: Metabolome analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Misspelling: C1C12; Note=Occasionally.

Derived from site: In situ; Skeletal muscle; UBERON=UBERON_0001134.

Cell type: Myoblast; CL=CL_0000056.

PubMed=563524; DOI=10.1038/270725a0

Yaffe D., Saxel O.

Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.

Nature 270:725-727(1977)

 

PubMed=8023908; DOI=10.1152/ajpcell.1994.266.6.C1795

McMahon D.K., Anderson P.A.W., Nassar R., Bunting J.B., Saba Z., Oakeley A.E., Malouf N.N.

C2C12 cells: biophysical, biochemical, and immunocytochemical properties.

Am. J. Physiol. 266:C1795-C1802(1994)

 

PubMed=15596414

Burattini S., Ferri P., Battistelli M., Curci R., Luchetti F., Falcieri E.

C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization.

Eur. J. Histochem. 48:223-233(2004)

 

PubMed=19298647; DOI=10.1186/1471-2121-10-20; PMCID=PMC2663541

Herrera B., Inman G.J.

A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.

BMC Cell Biol. 10:20.1-20.11(2009)

 

PubMed=19350625; DOI=10.1002/bit.22318

Fujita H., Shimizu K., Nagamori E.

Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V.

Biotechnol. Bioeng. 103:1034-1041(2009)

 

PubMed=20635352; DOI=10.1002/bit.22865

Fujita H., Endo A., Shimizu K., Nagamori E.

Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.

Biotechnol. Bioeng. 107:894-901(2010)

 

PubMed=22020321; DOI=10.1016/j.yexcr.2011.10.004; PMCID=PMC3541016

Humphrey G.W., Mekhedov E., Blank P.S., de Morree A., Pekkurnaz G., Nagaraju K., Zimmerberg J.

GREG cells, a dysferlin-deficient myogenic mouse cell line.

Exp. Cell Res. 318:127-135(2012)

 

PubMed=24735950; DOI=10.1016/j.scr.2014.03.004

Willkomm L., Schubert S., Jung R., Elsen M., Borde J., Gehlert S., Suhr F., Bloch W.

Lactate regulates myogenesis in C2C12 myoblasts in vitro.

Stem Cell Res. 12:742-753(2014)

 

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847.1-847.11(2014)

 

PubMed=25894527; DOI=10.1371/journal.pone.0121314; PMCID=PMC4404347

Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.

A mass spectrometric-derived cell surface protein atlas.

PLoS ONE 10:E0121314-E0121314(2015)