NCI-H28细胞

NCI-H28细胞NCI-H28细胞NCI-H28;人间皮瘤细胞

Cell line name NCI-H28

Synonyms H28; H-28; NCIH28; HUT-28; HUT 28; HuT 28; HUT28; Hut28; HuT28

Accession CVCL_1555

Resource Identification Initiative To cite this cell line use: NCI-H28 (RRID:CVCL_1555)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Microsatellite instability: Instable (MSI-low) (Sanger).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Caution: According to ATCC the ethnicity of the donor is White, but exome analysis finds it to be mostly of African lineage.

Caution: It is not clear if this cell line originate from an epithelioid or sarcomatoid mesothelioma as there are contradictory reports concerning its exact nature.

Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.

Sequence variations

Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (PubMed=16630136).

Gene deletion; HGNC; 2514; CTNNB1; Zygosity=Homozygous (PubMed=11070089; PubMed=11464291).

Mutation; HGNC; 950; BAP1; Simple; p.Arg146Alafs*48 (c.438-24_438-2del23); Zygosity=Homozygous (PubMed=21642991; PubMed=26011428).

Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (PubMed=1568228).

Mutation; HGNC; 12687; VHL; Simple; p.Leu89His (c.266T>A); ClinVar=VCV000376047; Zygosity=Homozygous (PubMed=8183553; DepMap=ACH-000648).

HLA typing Source: PubMed=26589293

Class I

HLA-A A*02:02,68:01

HLA-B B*35:118,53:02

HLA-C C*04:01,06:02

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 65.11

Native American 0.21

East Asian, North 3.2

East Asian, South 0

South Asian 0

European, North 16.44

European, South 15.05

Disease Pleural malignant mesothelioma (NCIt: C7376)

Pleural mesothelioma (ORDO: Orphanet_50251)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Sex of cell Male

Age at sampling 48Y

Category Cancer cell line

STR profile Source(s): ATCC=CRL-5820; Cosmic-CLP=908470; PubMed=25877200

 

Markers:

Amelogenin X,Y

CSF1PO 10,11

D2S1338 18,22

D3S1358 17

D5S818 11

D7S820 10,12

D8S1179 10,12

D13S317 11,13

D16S539 12,13

D18S51 19

D19S433 12,14.2

D21S11 29,30

FGA 19.2,21

Penta D 9,10

Penta E 10,16

TH01 7,9

TPOX 11

vWA 14,15

 

Run an STR similarity search on this cell line

Publications

PubMed=6272398; DOI=10.1126/science.6272398

Moody T.W., Pert C.B., Gazdar A.F., Carney D.N., Minna J.D.

High levels of intracellular bombesin characterize human small-cell lung carcinoma.

Science 214:1246-1248(1981)

 

PubMed=1568228

Metcalf R.A., Welsh J.A., Bennett W.P., Seddon M.B., Lehman T.A., Pelin-Enlund K., Linnainmaa K., Tammilehto L., Mattson K., Gerwin B.I., Harris C.C.

p53 and Kirsten-ras mutations in human mesothelioma cell lines.

Cancer Res. 52:2610-2615(1992)

 

PubMed=8183553

Sekido Y., Bader S.A., Latif F., Gnarra J.R., Gazdar A.F., Linehan W.M., Zbar B., Lerman M.I., Minna J.D.

Molecular analysis of the von Hippel-Lindau disease tumor suppressor gene in human lung cancer cell lines.

Oncogene 9:1599-1604(1994)

 

PubMed=8806092; DOI=10.1002/jcb.240630505

Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.

NCI-Navy Medical Oncology Branch cell line data base.

J. Cell. Biochem. Suppl. 24:32-91(1996)

 

PubMed=11030152; DOI=10.1038/sj.onc.1203815

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Oncogene 19:4632-4639(2000)

 

PubMed=11070089; DOI=10.1073/pnas.97.23.12776; PMCID=PMC18840

Calvo R., West J., Franklin W.A., Erickson P.F., Bemis L., Li E., Helfrich B., Bunn P.A. Jr., Roche J., Brambilla E., Rosell R., Gemmill R.M., Drabkin H.A.

Altered HOX and WNT7A expression in human lung cancer.

Proc. Natl. Acad. Sci. U.S.A. 97:12776-12781(2000)

 

PubMed=11464291; DOI=10.1038/sj.onc.1204557

Shigemitsu K., Sekido Y., Usami N., Mori S., Sato M., Horio Y., Hasegawa Y., Bader S.A., Gazdar A.F., Minna J.D., Hida T., Yoshioka H., Imaizumi M., Ueda Y., Takahashi M., Shimokata K.

Genetic alteration of the beta-catenin gene (CTNNB1) in human lung cancer and malignant mesothelioma and identification of a new 3p21.3 homozygous deletion.

Oncogene 20:4249-4257(2001)

 

PubMed=16630136; DOI=10.1111/j.1349-7006.2006.00184.x; PMCID=PMC11158456

Usami N., Fukui T., Kondo M., Taniguchi T., Yokoyama T., Mori S., Yokoi K., Horio Y., Shimokata K., Sekido Y., Hida T.

Establishment and characterization of four malignant pleural mesothelioma cell lines from Japanese patients.

Cancer Sci. 97:387-394(2006)

 

PubMed=17270034; DOI=10.1111/j.1349-7006.2006.00386.x; PMCID=PMC11158069

Taniguchi T., Karnan S., Fukui T., Yokoyama T., Tagawa H., Yokoi K., Ueda Y., Mitsudomi T., Horio Y., Hida T., Yatabe Y., Seto M., Sekido Y.

Genomic profiling of malignant pleural mesothelioma with array-based comparative genomic hybridization shows frequent non-random chromosomal alteration regions including JUN amplification on 1p32.

Cancer Sci. 98:438-446(2007)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

 

PubMed=21245096; DOI=10.1158/0008-5472.CAN-10-2164

Murakami H., Mizuno T., Taniguchi T., Fujii M., Ishiguro F., Fukui T., Akatsuka S., Horio Y., Hida T., Kondo Y., Toyokuni S., Osada H., Sekido Y.

LATS2 is a tumor suppressor gene of malignant mesothelioma.

Cancer Res. 71:873-883(2011)

 

PubMed=21642991; DOI=10.1038/ng.855; PMCID=PMC4643098

Bott M.J., Brevet M., Taylor B.S., Shimizu S., Ito T., Wang L., Creaney J., Lake R.A., Zakowski M.F., Reva B., Sander C., Delsite R., Powell S.N., Zhou Q., Shen R.-L., Olshen A.B., Rusch V.W., Ladanyi M.

The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma.

Nat. Genet. 43:668-672(2011)