EA.hy926细胞

EA.hy926细胞系、EA.hy926细胞株、EA.hy926细胞、EA.hy926细胞、EA.hy926 脐静脉细胞融合

Cell line name EA.hy 926

Synonyms EA. hy 926; EA hy 926; EA-hy926; EAhy 926; EAHY-926; EA.Hy926; EA.hy926; EAhy926; EaHy926; Eahy926

Accession CVCL_3901

Resource Identification Initiative To cite this cell line use: EA.hy 926 (RRID:CVCL_3901)

Comments Group: Space-flown cell line (cellonaut).

Characteristics: Produced by the fusion of A549/8 with human umbilical vein endothelial cells.

Doubling time: 12 hours (PubMed=6407019).

Omics: Transcriptome analysis by microarray.

Anecdotal: Have been flown in space on Shijian-10 and on the ISS in 2016 to study growth and secretion in microgravity (PubMed=28199884; PubMed=30108515; PubMed=30977987).

Misspelling: EA.hy 296; Note=Occasionally.

Misspelling: EA-hy 296; Note=Occasionally.

Misspelling: EA.hy296; Note=Occasionally.

Sequence variations

Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).

Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Parent: CVCL_IY87 (A549/8)

Children:

CVCL_C8WK (EA.hy926-EGFP) CVCL_C8VU (EA.hy926-Luc)

Sex of cell Male

Category Hybrid cell line

PubMed=7791520; DOI=10.1016/0024-3205(95)00227-W

Ahn K., Pan S., Beningo K., Hupe D.

A permanent human cell line (EA.hy926) preserves the characteristics of endothelin converting enzyme from primary human umbilical vein endothelial cells.

Life Sci. 56:2331-2341(1995)

 

PubMed=12453433; DOI=10.1006/mvre.2002.2434

Unger R.E., Krump-Konvalinkova V., Peters K., Kirkpatrick C.J.

In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.

Microvasc. Res. 64:384-397(2002)

 

PubMed=16575254; DOI=10.1097/01.mbc.0000220237.99843.a1

Boerma M., Burton G.R., Wang J.-R., Fink L.M., McGehee R.E. Jr., Hauer-Jensen M.

Comparative expression profiling in primary and immortalized endothelial cells: changes in gene expression in response to hydroxy methylglutaryl-coenzyme A reductase inhibition.

Blood Coagul. Fibrinolysis 17:173-180(2006)

 

PubMed=26345505; DOI=10.2144/000114326

Lieber D., Hochdorfer D., Stoehr D., Schubert A., Lotfi R., May T., Wirth D., Sinzger C.

A permanently growing human endothelial cell line supports productive infection with human cytomegalovirus under conditional cell growth arrest.

BioTechniques 59:127-136(2015)

 

PubMed=28199884; DOI=10.1016/j.biomaterials.2017.02.005

Pietsch J., Gass S., Nebuloni S., Echegoyen D., Riwaldt S., Baake C., Bauer J., Corydon T.J., Egli M., Infanger M., Grimm D.

Three-dimensional growth of human endothelial cells in an automated cell culture experiment container during the SpaceX CRS-8 ISS space mission -- the SPHEROIDS project.

Biomaterials 124:126-156(2017)

 

PubMed=30108515; DOI=10.3389/fphys.2018.01025; PMCID=PMC6079262

Li N., Wang C.-Z., Sun S.-J., Zhang C., Lu D.-Y., Chen Q., Long M.

Microgravity-induced alterations of inflammation-related mechanotransduction in endothelial cells on board SJ-10 satellite.

Front. Physiol. 9:1025.1-1025.15(2018)

 

PubMed=30977987; DOI=10.33594/000000071

Kruger M., Pietsch J., Bauer J., Kopp S., Carvalho D.T.O., Baatout S., Moreels M., Melnik D., Wehland M., Egli M., Sahana J., Kobbero S.D., Corydon T.J., Nebuloni S., Gass S., Evert M., Infanger M., Grimm D.

Growth of endothelial cells in space and in simulated microgravity -- a comparison on the secretory level.

Cell. Physiol. Biochem. 52:1039-1060(2019)