- ￥4500
- Sino Biological
- 北京
- 40592-V08H132
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant SARS-CoV-2 (BA.2.12.1) Spike RBD Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg
Coronavirus spike重组蛋白|Recombinant SARS-CoV-2 (BA.2.12.1) Spike RBD Protein (His Tag)（产品说明）
蛋白名称：SARS-CoV-2 (BA.2.12.1) Spike RBD Protein (His Tag) (HPLC-verified)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (BA.2.12.1) Spike RBD (YP_009724390.1, with mutations G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H)(Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.12.1).
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (BA.2.12.1) Spike RBD Protein (His Tag) (Cat:40592-V08H132), the EC50 is 8-30 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (BA.2.12.1) Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.72 kDa. As a result of glycosylation, it migrates as an approximately 35.86 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541
蛋白名称：SARS-CoV-2 (BA.2.12.1) Spike RBD Protein (His Tag) (HPLC-verified)
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (BA.2.12.1) Spike RBD (YP_009724390.1, with mutations G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H)(Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.12.1).
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
蛋白活性：Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (BA.2.12.1) Spike RBD Protein (His Tag) (Cat:40592-V08H132), the EC50 is 8-30 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (BA.2.12.1) Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.72 kDa. As a result of glycosylation, it migrates as an approximately 35.86 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541
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文献和实验该产品被引用文献
1, Shi D, et al. Structural Characteristics of Heparin Binding to SARS-CoV-2 Spike Protein RBD of Omicron Sub-Lineages BA.2.12.1, BA.4 and BA.5.Viruses, PubMed ID: 36560700
2, Poulakou G, et al. Anti-SARS-CoV-2 glyco-humanized polyclonal antibody XAV-19: phase II/III randomized placebo-controlled trial shows acceleration to recovery for mild to moderate patients with COVID-19.Frontiers in immunology, PubMed ID: 38694503
2, Poulakou G, et al. Anti-SARS-CoV-2 glyco-humanized polyclonal antibody XAV-19: phase II/III randomized placebo-controlled trial shows acceleration to recovery for mild to moderate patients with COVID-19.Frontiers in immunology, PubMed ID: 38694503
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Coronavirus spike重组蛋白|Recombinant SARS-CoV-2 (BA.2.12.1) Spike RBD Protein (His Tag)
￥4500
