草酸钾抗凝牛血

草酸钾抗凝牛血上海圻明生物优势供应，公司血清产品主要涵盖常规血清、血浆、红细胞、动物抗凝全血、无菌脱纤维全血、牛血清白蛋白、溶血素等产品等，大鼠小鼠品种可选，支持定制其他动物红细胞、血浆。
封闭系统无菌采血，无任何细菌、真菌、支原体、衣原体，不添加任何防腐剂、抗生素，适应与血平板生产、细菌培养、生物医学研究基础等。

血清保存注意事项：

1、需要保存的血清必须储存于-20℃ - 70℃ 低温冰箱中。4℃冰箱中保存时间切勿超过1个月。由于血清结冰时体积会增加约10%，因此，血清在冻入低温冰箱前，必须预留一定体积空间，否则易发生污染或玻璃瓶冻裂。
2、热灭活是指56℃，30分钟加热已解冻的血清。加热过程中须规则摇晃均匀。此热处理的目的是使血清中的补体成分活。除非必须，一般不建议作此热处理，因为热处理会造成血清沉淀物显著增多，而且还会影响血清的质量。补体参与反应有细胞毒作用，平滑肌细胞收缩，肥大细胞和血小板释放组胺，增强吞噬作用，促进淋巴细胞和巨噬细胞发生化学趋化和活化。
3、瓶装血清解冻需采用逐步解冻法：-20℃ 至 -70℃ 低温冰箱中的血清放入4℃冰箱中溶解1天。然后移入室温，待全部溶解后再分装，在溶解过程中需不断轻轻摇晃均匀(小心勿造成气泡)，使温度与成分均一，减少沉淀的发生。切勿直接将血清从-20℃进入37℃解冻，这样因温度改变太大，容易造成蛋白质凝集而出现沉淀。
4、一般厂商提供的血清为无菌，无需再过滤除菌。如发现血清有悬浮物，则可将血清加入培养液内一起过滤,切勿直接过滤血清。
5、血清中的沉淀物絮状物主要是血清中的脂蛋白变性及解冻后血清中纤维蛋白造成，这些絮状物不会影响血清本身的质量，可用离心3000rpm, 5分钟去除，也可不用处理，显微镜下"小黑点":经过热处理过的血清，沉淀物的形成会显著增多。有些沉淀物在显微镜下观察象"小黑点",常误认为血清受污染。一般情况下，此小黑点不会影响细胞生长，但如果怀疑血清质量，则应立即停止使用，更换另一批号的血清。
6、切勿将血清在37℃放置太久，否则血清会变得浑浊。同时血清中的有效成分会破会而影响血清质量。

Precautions for serum preservation:

1, need to save the serum must be stored in -20 ℃ - 70 ℃ cryogenic refrigerator. 4 ℃ refrigerator storage time should not exceed 1 month. Because the serum will increase in volume by about 10% when frozen, therefore, serum must be reserved for a certain volume of space before freezing into the cryogenic refrigerator, otherwise it is easy to contamination or freeze cracking of the glass vials.
2, thermal inactivation refers to 56 ℃, 30 minutes heating has been thawed serum. Heating process must be rule shaking evenly. The purpose of this heat treatment is to make the complement components in the serum live. This heat treatment is generally not recommended unless it is necessary because it causes a significant increase in serum precipitates and also affects the quality of the serum. Complement involved in the reaction has a cytotoxic effect, smooth muscle cell contraction, mast cells and platelets release histamine, enhance phagocytosis, promote lymphocytes and macrophages chemotaxis and activation.
3, bottled serum thawing need to use step-by-step thawing method: -20 ° C to -70 ° C cryogenic refrigerator serum into the 4 ° C refrigerator to dissolve 1 day. Then move to room temperature, to be completely dissolved and then divided, in the process of dissolution need to be constantly shaken gently and evenly (careful not to cause air bubbles), so that the temperature and composition of the uniform, to reduce the occurrence of precipitation. Do not thaw the serum directly from -20 ℃ into 37 ℃, so that the temperature change is too large, easy to cause protein agglutination and precipitation.
4. Generally, the serum provided by the manufacturer is sterile and does not need to be filtered for sterilization. If the serum is found to be suspended, the serum can be added to the culture medium and filtered together, do not filter the serum directly.
5, the precipitate in the serum flocculent is mainly caused by the denaturation of lipoproteins in the serum and fibrin in the thawed serum, these flocculent will not affect the quality of the serum itself, can be removed by centrifugation at 3000rpm for 5 minutes, or can be left unprocessed, microscopic “small black dots”: the formation of precipitates after heat treatment of the serum, will increase significantly. Some precipitates are observed under the microscope as “small black spots”, which are often mistaken for contaminated serum. Under normal circumstances, this small black spots will not affect cell growth, but if you suspect the quality of serum, you should immediately stop using, replace another batch of serum.
6, do not leave the serum at 37 ℃ for too long, otherwise the serum will become turbid. At the same time, the active ingredients in the serum will break down and affect the quality of the serum.

草酸钾抗凝牛血收到后请于-20℃冰箱放置。
建议收到后及时使用，有效期内开封后2周内用完。
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