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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
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- 保修期：
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货号：T_70110-800-168
中文名称：
英文名称：2ML POLYPROPYLENE VIAL 1000/CS
货期：热销品现货，其他请咨询
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文献和实验Polyclonal and Monoclonal Antibodies
Maintenance of the cells: Stock solutions: IMDM : Fetal Calf Serum- Transferrin: Iron saturated. 1000X stock = 1 mg/ml HT supps: 50X from Sigma H0137 ( Store at -20 C ). 2-Mercaptoethanol: 1000X stock (5 x 10 -2). (Store
ES Cell Culture and Manipulation
volume in ml = total # of cells). Centrifuge cells and resuspend in 2.4ml DMEM, divide into 3 aliquots of 800 µl each (~2 x 107 cells/800µl. Add 30µg sterile linearized targeting DNA each to 2 tubes (1 for G418 selection, 1 for G418/gancyclovir
ES Cell Culture and Manipulation
ribonucleosides 1/100 volume ß-ME stock (stock is 7µl ß-ME in 10ml DMEM) 1:1000 dilution of LIF (add 500 µl 720LIFD cell conditioned media to 500ml media) Thawing frozen vials of ES cells Thaw vial rapidly at 37°C, rinse outside of vial
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