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- BOSTER
- A31819-100ul
- 中国
- 2025年07月16日
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产品概况
| 货号 | A31819 |
|---|---|
| 产品名称 | Anti-RTL3 Antibody |
| 基因名 | RTL3 |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 免疫原 | E.coli-derived human RTL3 recombinant protein (Position: Q168-Q475). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Retrotransposon Gag like 3 is a protein that in humans is encoded by the RTL3 gene. This gene is a member of a family of gag-related retrotransposon genes. These genes appear to have lost the ability to retrotranspose; however, their open reading frames have remained intact, which may indicate that these genes have acquired new functions in the cell. |
| 研究类别 | 1. Jürgen Brandt, Schrauth, S. , Veith, A. M. , Froschauer, A. , Haneke, T. , & Schultheis, C. , et al. (2005). Transposable elements as a source of genetic innovation: expression and evolution of a family of retrotransposon-derived neogenes in mammals. Gene, 345(1), 101-111.2. Brandt, J. , Veith, A. M. , & Volff, J. N. . (2005). A family of neofunctionalized ty3/gypsy retrotransposon genes in mammalian genomes. Cytogenetic & Genome Research, 110(1-4), 307-317.3. Ota, T. , Suzuki, Y. , Nishikawa, T. , Otsuki, T. , & Sugano, S. . (2004). Complete sequencing and characterization of 21,243 full-length human cdnas. Nature Genetics, 36(1), 40-45. |
| Uniprot ID | RTL3: Q8N8U3 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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文献和实验Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
a simple ELISA method to detect human anti-antibody response.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
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