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文献和实验Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
9 (TG30) monoclonal antibody (Millipore, cat. no. MAB4427) Mouse anti‐GCTM2 monoclonal antibody (0.4 mg/ml; kind gift from Martin Pera)—a commercially available alternative, which detects
Coupling Peptides To Resin For Affinity Purification
. The amino resin will react with an NHS ester, whereas the original affigel and the iodoacetate will not. Take an aliquot (50ul) of resin at each step. Resuspend in 100 ul of buffer. Add 1 ul of 0.1 M NHS fluoroscein or NHS-rhodamine in DMSO. Incubate 5' RT
with a broad spectrum of cytokeratins of different molecular weights (including cytokeratins 5, 6, 8, 17, and possibly 19), i.e. it is considered a "pan-cytokeratin antibody" (although this antibody may not cover the cytokeratins of very low M.W. or very high M
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