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- 规格：
2ug
Murine Wnt-3a (2ug)
Wnt-3a 315-20
别名：Wingless-type MMTV (mouse mammary tumor virus)integration site family member 3a
描述：Wnt-3a属于信号蛋白的Wnt家族，在维持胚胎和成体组织的完整性中发挥着重要的作用。Wnt-3a主要沿跨越中枢神经系统(CNS)重叠区域的背侧中线表达。Wnt-3a信号在包括胚胎成型、细胞决定、细胞增殖、CNS发育和细胞骨架形成等多种形态发生过程中不可或缺。与该家族的其它成员相似，Wnt-3a含有一个高度保守的脂类修饰的半胱氨酸富集域，该区域为细胞信号传导所必需。经典Wnt通路在生物体的胚胎形成和发育以及干细胞的更新和分化中起着重要作用，Wnt通路由胞外Wnt配体同时结合一个七次跨膜受体（Frizzled）和低密度脂蛋白受体相关蛋白5/6（Lrp5/6）而被激活，而体内的E3泛素连接酶ZNRF3/RNF43持续泛素化Wnt受体Frizzled，使Frizzled被降解，从而抑制了Wnt信号通路，而当R-spondin存在时，R-spondin与LGR和ZNRF3/RNF43结合形成复合物，Frizzled的降解被抑制，蛋白水平上升，Wnt通路被又被激活。因此在类器官培养体系中往往Wnt-3a 和R-Spondin两种因子都需要添加。
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Product Details |
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Size |
2 µg |
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Species |
Mouse |
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Published Species |
Rat, Non-human primate, Human, Mouse, Chicken |
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Expression system |
Mammalian cells |
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Amino acid sequence |
SYPIWWSLAV GPQYSSLSTQ PILCASIPGL VPKQLRFCRN YVEIMPSVAE GVKAGIQECQ HQFRGRRWNC TTVSNSLAIF GPVLDKATRE SAFVHAIASA GVAFAVTRSC AEGSAAICGC SSRLQGSPGE GWKWGGCSED IEFGGMVSRE FADARENRPD ARSAMNRHNN EAGRQAIASH MHLKCKCHGL SGSCEVKTCW WSQPDFRTIG DFLKDKYDSA SEMVVEKHRE SRGWVETLRP RYTYFKVPTE RDLVYYEASP NFCEPNPETG SFGTRDRTCN VSSHGIDGCD LLCCGRGHNA RTERRREKCH CVFHWCCYVS CQECTRVYDV HTCK |
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Molecular weight |
37.4 kDa |
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Class |
Recombinant |
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Type |
Protein |
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Purity |
75% by SDS-PAGE gel and HPLC analyses. |
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Endotoxin concentration |
<2 EU/µg |
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Activity |
Determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50 for this effect is 0.4-0.6 ng/ml. |
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Conjugate |
Unconjugated |
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Form |
Lyophilized |
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Purification |
purified |
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Contains |
no preservative |
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Storage conditions |
-20°C |
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Applications |
Tested Dilution |
Publications |
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Functional Assay (Functional) |
Assay-dependent |
1 Publication |
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In vitro Assay (IV) |
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80 Publications |
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Miscellaneous PubMed (Misc) |
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19 Publications |
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Product Specific Information
Recombinant Murine Wnt-3a is a monomeric glycoprotein containing 334 amino acid residues. Due to glycosylation, the
Murine Wnt-3a migrates at an apparent molecular weight of approximately 38.0-41.0 kDa by SDS-PAGE analysis under non- reducing conditions.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis
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Mouse Wnt-3a Protein (315-20-2UG) in Functional |
| Bioassay analysis of Mouse Wnt-3a Recombinant Protein, PeproTech® (Product # 315-20-10UG). |
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文献和实验[1]Liu F, Lin Q, Shen S, Li Z, Xie X, Cheng Q, Wang L, Long Y, Wang J, Liu L. Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration. iScience. 2024 May 3;27(6):109888. doi: 10.1016/j.isci.2024.109888. PMID: 38947517; PMCID: PMC11214297.
[2]Xu X, Wang J, Du S, Shen X, Lian J, Zhou J, Wang M, Feng W, Lv Z, Zhu J, Jin L, Sun H, Wu L, Wang X, Qiu H, Wang W, Teng H, Wang Y, Huang Z. Yes-associated protein regulates glutamate homeostasis through promoting the expression of excitatory amino acid transporter-2 in astrocytes via β-catenin signaling. Glia. 2023 May;71(5):1197-1216. doi: 10.1002/glia.24332. Epub 2023 Jan 8. PMID: 36617748.
[3]Tejeda-Muñoz N, Morselli M, Moriyama Y, Sheladiya P, Pellegrini M, De Robertis EM. Canonical Wnt signaling induces focal adhesion and Integrin beta-1 endocytosis. iScience. 2022 Mar 19;25(4):104123. doi: 10.1016/j.isci.2022.104123. PMID: 35402867; PMCID: PMC8987407.
[4]Sufi J, Qin X, Rodriguez FC, Bu YJ, Vlckova P, Zapatero MR, Nitz M, Tape CJ. Multiplexed single-cell analysis of organoid signaling networks. Nat Protoc. 2021 Oct;16(10):4897-4918. doi: 10.1038/s41596-021-00603-4. Epub 2021 Sep 8. PMID: 34497385.
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