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- 详细信息
- 技术资料
- 免疫原:
A phospho specific peptide corresponding to residues surrounding S5 of human POLR2A carboxy-terminal domain (CTD) heptapeptide repeat YSPTSPS.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX03215
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
0.25 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
RPB1 CTD (phospho Ser5)
- 抗体英文名:
RPB1 CTD (phospho Ser5) antibody [GT1303]
- 抗体名:
RPB1 CTD (phospho Ser5) 抗体 [GT1303]
- 规格:
100 μl
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB1 CTD (phospho Ser5) antibody [GT1303] (GTX03215) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
WB analysis of C2C12 cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03215 RPB1 CTD (phospho Ser5) antibody [GT1303].
Dilution : 1:1000
Loading : 25μg per lane
WB analysis of MCF7 cells were treated by Doxorubicin (0.5 uM) at 37ºC for 24 hours or treated by nocodazole (50 ng/mL) at 37ºC for 20 hours or treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03215 RPB1 CTD (phospho Ser5) antibody [GT1303].
Dilution : 1:1000
Loading : 25μg per lane
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