DSN可以精确区分10-12bp的DNA-DNA双链的单核苷酸错配,dsDNA大于10bp、DNA-RNA大于15 bp 时DSN才能产生切割活性。
广泛用于全长cDNA 富集后均一化操作、基于二代测序(NGS)的转录组文库构建等,DSN还用于基因组DNA均一化、cDNA缺失和核糖体cDNA缺失、cDNA差减、SNP检测、repeat-free FISH 探针,miRNA的多重荧光检测,端粒末端定量检测等。
cat.# EA001; EA002; EA003
Selectively cleaves ds DNA and DNA in DNA-RNA hybrid duplexes
- Discriminates between perfectly and nonperfectly matched short duplexes
- Inactive towards ss DNA and RNA
- Thermostable
- Inhibited by EDTA