Wild-type (WT) and Vimentin knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.
Vimentin antibody [HL1506] detects Vimentin protein at cytoskeleton and cell junction by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat lymph node.Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min