Background: Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER.
Immunogen: Recombinant full length protein of active Sar1a.
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration: 1 mg/ml
Host Species: Mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Preservative: No
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Sar1 antibody recognizes active Sar1 of vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:
Sar1 Activation Assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No. 26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody (Cat. No. ).