pEF1/myc-His A

别名：pEF1/myc-HisA

质粒类型：哺乳动物表达载体

表达水平：高拷贝

启动子：EF-1a

载体大小：6165 bp 

5' 测序引物及序列：T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'

载体标签：6X His, myc

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Neomycin

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

（1）Human elongation factor 1α subunit (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996; Mizushima and Nagata, 1990).

（2）Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide.

（3）Neomycin resistance gene for selection of stable cell lines.

（4）Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS7).

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

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