The base medium for this cell line is DMEM:F12 Medium.To make the complete growth medium, add the following components to the base medium:extra 10 mM HEPES (for a final conc. of 25 mM)10 ng/ml cholera toxin0.005 mg/ml insulin0.005 mg/ml transferrin100 ng/ml hydrocortisone20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)Fetal Bovine Serum, 10% final conc.