Ammonium sulfate is a widely used reagent in molecular biology and chromatography. It is useful in the isolation and purification of enzymes and proteins. It is used for the precipitation or fractionation of proteins or for purification of antibodies.It is also useful for crystallographic analysis of nucleic acids and proteins. Ammonium sulfate is also widely used in HPLC of proteins, such as in hydrophobic interaction chromatography. Ammonium sulfate is used in long PCR buffer, in PCR lysis solution, and in secondstrand cDNA synthesis. Also it is used for the HPLC analysis of phospholipids.
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