Whole-Genome Allelotyping Using Laser Microdissected Tissue
Laser-based microdissection technologies have been recently developed and applied to procure homogenous populations of tumor cells from paraffin-embedded and frozen tissue sections. When combined with whole-genomic amplification techniques, sufficient amounts of D ...
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Immunochromatographic Lateral Flow Strip Tests
The immunochromatographic lateral flow strip test is a one-step test that facilitates low-cost, rapid identification of various analytes at the point of care. We have developed lateral flow strip tests for the specific qualitative or semiquantitative detection of antigens, antibo ...
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高密度光纤芯片技术及其在功能基因组学中的应用
DNA微阵列技术的发展带来了基因表达研究方法上的一场革命。传统的Northern blots或RT-PCR方法只能逐一地研究单个基因的表达,而DNA微阵列技术可以同时例行检测成千上万个基因表达水平的变化,在微芯片上置入寡核苷酸探针或相应于mRNA序列的cDNA,与细胞cDNA或cRNA进行杂交,可以纵观细胞的整体基因表达而不是从前的一个局部。例如,毒理学家可以利用基因表达微阵列芯片快速地对潜在的有 ...
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Bioluminescence Reporter Gene-Based Detection of MicroRNAs
MicroRNAs (miRNAs) are an abundant class of small noncoding RNA molecules that inhibit the expression of cognate genes in multicellular organisms. These small RNAs have been demonstrated to play crucial roles in a variety of biological processes including cell differentiation, pro ...
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