Cell-Based Bioluminescence Screening Assays
Drug screening is an essential and widely used technique for drug discovery in various biomedical fields notably in oncology. Here we describe a functional screening assay based on the bioluminescence detection of a secreted luciferase for monitoring cell viability of cancer cells in a h ...
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CCD Camera Detection of HIV Infection
Rapid and precise quantification of the infectivity of HIV is important for molecular virologic studies, as well as for measuring the activities of antiviral drugs and neutralizing antibodies. An indicator cell line, a CCD camera, and image-analysis software were used to quantify HIV inf ...
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Photomultiplier Tubes in Biosensors
Photomultiplier tubes (PMT) are widely used for the weak light detection in some types of biosensors. A light detection system for biosensors based on PMT generally contains optic fibers, PMT, and filters. Basic principles of those accessories were provided in this chapter. The guides to sele ...
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Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells
Adenosine 5′-triphosphate (ATP) is the “energy currency” of organisms and plays central roles in bioenergetics, whereby its level is used to evaluate cell viability, proliferation, death, and energy transmission. In this chapter, we describe an improved and efficient method for extrac ...
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