摘抄:
Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling Technology (reference 9386). It recognizes phosphorylated threonine residues in a manner largely independent of the surrounding amino-acid sequence. The antibody is phospho-specific but does not cross-react with phospho-serine-containing sequences. The anti-phosphothreonine monoclonal antibody is labeled with Eu3+ Cryptate by Cisbio international. Specific activity of the conjugate ranges from 4 to 7 Cryptates per antibody. This conjugate is supplied in 50 mM hepes buff er pH 7.0, 0.1% bovine serum albumin (BSA) protease free.
The concentration of this conjugate has been calibrated in order to obtain a 620 nm signal within the 30,000-50,000 count range. This calibration was run in a 384-well low volume plate format using a final volume of 20 μL, a buffer containing 0.1% BSA and 0.4M KF, and a RUBYstar reader (BMG LABTECH). A recommended amount of antibody per well is specified in the product information sheet attached to the product. On this basis, each vial enables the assessment of 500 tests. Actual amount per well will be dependent on optimized assay conditions and miniaturization.
摘抄:
Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling Technology (reference 9386). It recognizes phosphorylated threonine residues in a manner largely independent of the surrounding amino-acid sequence. The antibody is phospho-specific but does not cross-react with phospho-serine-containing sequences. The anti-phosphothreonine monoclonal antibody is labeled with Eu3+ Cryptate by Cisbio international. Specific activity of the conjugate ranges from 4 to 7 Cryptates per antibody. This conjugate is supplied in 50 mM hepes buff er pH 7.0, 0.1% bovine serum albumin (BSA) protease free.
The concentration of this conjugate has been calibrated in order to obtain a 620 nm signal within the 30,000-50,000 count range. This calibration was run in a 384-well low volume plate format using a final volume of 20 μL, a buffer containing 0.1% BSA and 0.4M KF, and a RUBYstar reader (BMG LABTECH). A recommended amount of antibody per well is specified in the product information sheet attached to the product. On this basis, each vial enables the assessment of 500 tests. Actual amount per well will be dependent on optimized assay conditions and miniaturization.