The complete sequencing workflow of the Genome Sequencer FLX System comprises four main steps, leading from purified DNA to analyzed results. These basic steps include:
Generation of a single-stranded template DNA library
Emulsion-based clonal amplification of the library
Data generation via sequencing-by-synthesis
Data analysis using different bioinformatics tools
For an overview of the entire sequencing workflow, please see the Genome Sequencer FLX Multimedia Presentation.