在所有生物功能中,蛋白质复合物的功能占主导地位;在所有细胞功能中,蛋白质间的相互作用是最基本的活动。研究蛋白质组相互作用的方法主要有酵母双杂交技术(Y2H)、亲和纯化(AP)、化学交联偶联质谱CXMS)等方法。其中 AP 技术使用特别设计的纯化标签在非常接近于生理条件的情况下,能捕捉出特定的蛋白质及其分子伴侣。SWATH 和 TAP 技术联合使用确定蛋白复合体的成分是 SWATH 定量方法最常用的应用之一,目前已经非常成熟。
金开瑞生物高精度质谱平台,开发了一套从实验到生物信息分析的 AP-SWATH 流程,采用 TAP 技术将目标靶蛋白的相互作用蛋白特异性富集,然后结合具有高灵敏度、高准确度、高通量等特点的SWATH 定量,能够高特异性的准确富集到靶蛋白的特异性相互作用蛋白,实现复合蛋白质的准确鉴定。
差异蛋白网络互作:基于 STRING 数据库的 PPI 分析,基于 Cytoscape 的可视化展示。
五、SWATH技术近期发表的相关文献 Quantifying protein interaction dynamics by SWATH mass spectrometry: application to the 14-3-3 system.NM.2013 Mapping differential interactomes by affinity purification coupled with data-independent mass spectrometry acquisition.NM .2013 Quantitative measurements of N-linked glycoproteins in human plasma by SWATH-MS. Proteomics.2013 Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. MCP.2014 SWATH™- and iTRAQ-based quantitative proteomic analyses reveal an overexpression and biological relevance of CD109 in advanced NSCLC.JP.2014 Variation and quantification among a target set of phosphopeptides in human plasma by multiple reaction monitoring and SWATH-MS2 data-independent acquisition.Electrophoresis.2014 Expansion of the ion library for mining SWATH-MS data through fractionation proteomics. AC.2014 Quantitative proteomics by SWATH-MS reveals altered expression of nucleic acid binding and regulatory proteins in HIV-1-infected macrophages. JPR .2014 Reproducible and consistent quantification of the Saccharomyces cerevisiae proteome by SWATH-MS.MCP.2015 SWATH enables precise label-free quantification on proteome-scale. Proteomics.2015 Quantitative variability of 342 plasma proteins in a human twin population.MSB.2015 SWATH Analysis for Characterization and Quantification of Histone Post-translational Modifications. MCP.2015