因工作需要,想查一下最原始的有关PCR论文,在PubMed上,用(“Polymerase chain reaction”[All])搜索了1984-1987年的论文,结果有11篇,其中最早的在1986年发表,共3篇,具体如下:
9: Saiki RK, Bugawan TL, Horn GT, Mullis KB, Erlich HA. Related Articles, Links
Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes.
Nature. 1986 Nov 13-19;324(6093):163-6.
PMID: 3785382 [PubMed - indexed for MEDLINE]
10: Scharf SJ, Horn GT, Erlich HA. Related Articles, Links
Direct cloning and sequence analysis of enzymatically amplified genomic sequences.
Science. 1986 Sep 5;233(4768):1076-8.
PMID: 3461561 [PubMed - indexed for MEDLINE]
11: Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H. Related Articles, Links
Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.
Cold Spring Harb Symp Quant Biol. 1986;51 Pt 1:263-73. No abstract available.
PMID: 3472723 [PubMed - indexed for MEDLINE]