大家好,由于我的基础不太扎实,希望各位战友能告诉我,这种是什么实验方法。简单说明其过程。1,transgenic mice that overexpress Runx2 under the control of a 2.3-kb mouse Col1a1 promoter。2,In dn-Runx2 transgenic mice under the control of the same
2.3-kb mouse Col1a1 promoter。
问题一,是上面是通过什么方法进行基因改造的,望战友简单说说其过程是怎样的?
问题二,Runx2是一种成骨细胞的一种转录因子。是促进骨髓干细胞向成骨细胞分化和促进成骨 细胞特异蛋白的表达。问题是dn-Runx2是什么东西?它是生物或细胞本来就存在的,还是人为改造的呢?它的中文名应该叫什么?
问题表述的不好希望各位站友原谅,同时希望大家帮帮忙,祝大家国庆快乐!
原文:transgenic mice that overexpress Runx2 under
the control of a 2.3-kb mouse Col1a1 promoter, which
directs reporter gene expression to immature and mature
osteoblasts, show osteopenia with multiple fractures (Liu et
al. 2001; Geoffroy et al. 2002; Kanatani et al. 2006).
In dn-Runx2 transgenic mice under the control of the same
2.3-kb mouse Col1a1 promoter, the volume of the trabecular
bone is increased, and the expression of major bone matrix
protein genes including Col1a1, Spp1, and Bglap, is not
significantly affected compared with those in wild-type mice。