BEBP02-054Q LONZA X-VIVO 15  免疫细胞无血清培养基

BEBP02-054Q LONZA X-VIVO 15 免疫细胞无血清培养基

北京云肽生物科技代理的LONZA X-VIVO 15 免疫细胞培养基（货号：BEBP02-054Q），作为LONZA最畅销的无血清培养基，可为免疫细胞提供营养平衡完善的体外培养环境，已成功应用于T细胞、造血干细胞、单核细胞、巨噬细胞、CIK、自然杀伤细胞、外周血淋巴细胞、树突状细胞、淋巴因子激活杀伤细胞、肿瘤浸润淋巴细胞、粒细胞的培养中。



LONZA X-VIVO 15 培养基，不含外源生长因子、人工细胞增殖刺激因子、蛋白激酶C刺激剂，所有成分明确，含有重组人胰岛素、重组人转铁蛋白和白蛋白，并针对肿瘤浸润淋巴细胞（TIL）的体外无血清培养进行了优化，近年来在众多CAR-T研究中得到使用。




Serum-free media outperforms serum-based media and maintains phenotype, with secondary suppliers for cytokines.
T-cells (1x106) were stimulated with CD3/CD28 Dynabeads® in both 6-well plates (5 ml) and a closed system breathable vessel
(35 ml) with different media. The serum-free X-VIVO media supported the largest expansion of T-cells, comparable to RPMI
 supplemented with human serum in both open and closed systems and outperforming several serum-free media alternatives 

LONZA X-VIVO 15 已在美国FDA进行备案。 生产用X-VIVO 15培养基由拥有质量管理认证的欧洲或美国工厂生产，严格按照cGMP标准，不含抗生素、不含酚红，且全部标记为FFM (For Further Manufacture)。用于科研及研发用途的X-VIVO 15培养基，添加抗生素及酚红，并标记为RUO(Research Use Only)。

瑞士LONZA集团，在全球拥有100余个生产基地和办事处，全球员工14500余人，是著名的生物技术、制药、特殊成分供应商，多年来为基因治疗研究、过继免疫治疗研究及其他细胞疗法基础研究开发了众多研究工具。



LONZA X-VIVO 15 相关文献     

1. Highly efficient therapeutic gene editing of human hematopoietic stem cells.(NatureMedicine,2019)
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12. A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.(Nature Genetics,2016,49(2):193-203)