71186-3

YeastBuster^TM Protein Extraction Reagent
Description
YeastBuster Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The proprietary formulation utilizes a mix of mild detergents and protein stabilization buffer that, when combined with tris(hydroxypropyl)phosphine (THP) reducing agent, effectively releases protein from yeast cells (1). This powerful combination eliminates inconsistencies associated with tedious mechanical disruption of yeast cells using glass bead abrasives, ultrasonication, pressure disruption, or enzymatic disruption with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, Schizosaccharomyces pombe strains, as well as with filamentous fungi and plant cells (2-3). YeastBuster Protein Extraction Reagent plus Benzonase®  Nuclease is an efficient combination for gently releasing target proteins and markedly reducing extract viscosity prior to downstream processing. Yeast cells suspended in YeastBuster Reagent with Benzonase Nuclease are briefly incubated while soluble proteins are released and nucleic acids digested. Insoluble protein and cell debris are easily removed by centrifugation. The resulting low viscosity extract contains soluble proteins ready for additional purification or analysis. In addition to higher total protein yields in crude extracts and recovery of enzymatically active proteins, the extracts are fully compatible with GST•Bind™ and Ni-NTA His•Bind®  immobilized metal affinity chromatography (IMAC) purification methods and reporter assays [e.g., GST•Tag™ Assay Kit (Cat. No. 70532-3), BetaRed™ β-Gal Assay Kit (Cat. No. 70978-3)]. For applications that require no reducing agent, YeastBuster will also lyse yeast cells in early log phase without the addition of THP; however, total protein extraction efficiency is decreased under these conditions