（英）539779-亚细胞蛋白质组抽提试剂盒说明书

1. Introduction
ProteoExtract™ Complete Mammalian Proteome Extraction Kit is designed to extract total proteins from mamalian tissue culture cells or mammalian tissue. Two-dimensional-electrophoresis (2DGE) remains the highest resolution technique for protein separation when complex samples need to be characterized, as in proteomics. Pretreatment of samples for isoelectric focusing (IEF) followed by polyacrylamide gel electrophoresis (SDS-PAGE) involves solubilization, denaturation and reduction to completely break any interactions between the proteins and to remove non-protein sample components. Ideally, to avoid protein losses, one would achieve complete sample solubilization in a single step and thus eliminate unnecessary handling. The prerequisite of a successful proteome analysis is a standardized and reproducible sample preparation procedure. The Complete Mammalian ProteoExtract™ Kit (C-PEK) allows highly reproducible sample preparation from both tissue culture cells and tissues in two steps in a microcentrifuge tube minimizing protein loss. Extraction with C-PEK results in a total protein sample solubilized in the presence of ultrapure chemicals that is ready for two-dimensional separation. The complete cell extract is ideally suited for overview gels and for fast screening of the influence of experimental parameters on protein expression or modification by means of e.g. 2DE-Western-Blotting or autoradiography. A representative 2D gel of proteins extracted from a tissue culture cell line is shown in figure 1.