推荐产品
公司新闻/正文
贺北京中日友好医院应用PriCells产品/技术服务发表文章
人阅读 发布时间:2015-04-20 10:56
贺北京中日友好医院应用PriCells产品/技术服务发表文章
Biochem Biophys Res Commun. 2015 Mar 13;458(3):674-80. doi: 10.1016/j.bbrc.2015.02.022. Epub 2015 Feb 13.
Xu S1, Jiang Y1, Wang H1, Wang Z1, Liu H1, Peng L1, Fang Q1, Deng T1, You J1, Zhou X2, Zhang W3, Lou J4.
1Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, 100029 PR China.
2Department of Urology, China-Japan Friendship Hospital, Beijing, 100029 PR China.
3Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, 100029 PR China. Electronic address: zwj-72@163.com.
4Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, 100029 PR China. Electronic address: Lou.j@mail.com.
Abstract
Type 1 diabetes mellitus (T1DM) is characterized by the deficiencies of insulin and C-peptide. Mounting evidences have proved the beneficial effects of C-peptide on the renal function in T1DM. However, it is still controversial about the roles of C-peptide in T2DM nephropathy since the level of C-peptide fluctuates greatly at different stages of T2DM. In the present study, we found that the serum C-peptide concentration was much lower in GK rats with diabetic nephropathy than that in normal counterparts. A sustained supplementation of C-peptide at a physiological level could ameliorate urinary albumin, independent of blood glucose control. C-peptide treatment improved glomerulosclerosis and podocyte morphology and reduced the thickness of glomerular basement membrane as compared with saline treatment control. Moreover, it decreased fibronectin synthesis in diabetic glomeruli and in cultured rat mesangial cells accompanied by a down-regulation of RAGE and an up-regulation of PKA. Interestingly, H-89, a PKA inhibitor, could reverse the inhibition effect of C-peptide on fibronectin production in cultured mesangial cells. These findings suggest that C-peptide level is low in T2DM rats with nephropathy and a treatment with a physiological dose of C-peptide can prevent renal injury in diabetic GK rats.
RAT-CELL-0020;PriCells
RAT-CELL-0020;PriCells