贺武汉市中心医院中心实验室应用PriCells产品/技术服务发表文章

 

Huaier suppresses proliferation and induces apoptosis in human pulmonary cancer cells via upregulation of miR-26b-5p

 

FEBS Lett. 2014 Jun 5;588(12):2107-14.

doi: 10.1016/j.febslet.2014.04.044. Epub 2014 May 8.

 

Tangwei Wu^a,b,1, Weiqun Chen ^a,c,d,1, Shuiyi Liu ^d,1, Hongda Lu ^d,e, Hui Wang ^a, Deyong Kong ^a,

Xiaodong Huang ^c,d, Qinzhi Kong ^c,d,e, Yong Ning ^b,⇑, Zhongxin Lu ^a,c,d,⇑

 

a Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China

b School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China

c Department of Central Laboratory, Central Hospital of Wuhan, Wuhan 430014, China

d Cancer Research Institute of Wuhan, Wuhan 430014, China

e Department of Oncology, Central Hospital of Wuhan, Wuhan 430014, China

 

Abstract

Various studies have reported that Huaier possesses anti-tumor effects. However, the mechanisms are not completely elucidated. Here, we found 66 differentially expressed miRNAs in Huaier-treated pulmonary adenocarcinoma A549 cells, with upregulation of miR-26b-5p. Transfection of A549 cells with miR-26b-5p mimic inhibited proliferation and induced apoptosis, while transfection of Huaier-treated A549 cells with a miR-26b-5p inhibitor reversed the effects of Huaier. EZH2 was verified as the target of miR-26b-5p. Thus, our findings indicate that Huaier might suppress proliferation and induce apoptosis in lung cancer cells via a miR-26b-5p-EZH2-mediated approach, which provides a new perspective for understanding the anti-tumor effects of Huaier.

 

HUM-RNA-0007，PriCells