贺南京中医药大学应用PriCells产品/技术服务发表文章

 

Integrating microRNA and mRNA expression profiles in response to radiation-induced injury in rat lung

 

Radiation Oncology 2014, 9:111  

doi:10.1186/1748-717X-9-111

 

Ling Xie, Jundong Zhou, Shuyu Zhang, Qing Chen, Rensheng Lai, Weiqun Ding, ChuanJun Song, XingJun Meng and Jinchang Wu

 

¹Nan Jing Medical University, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China

² Department of Pathology, The Affiliated Hospital of Chinese Medicine University of Nanjing, Nanjing 210029, China

³ School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China

⁴ The Core Laboratory of Suzhou Cancer Center and Department of Radiotherapy of Suzhou Municipal Hospital, Suzhou 215001, China

⁵ Department of Pathology, University of Oklahoma Health Science Center, Oklahoma City, OKC, USA

 

 

Abstract

Purpose: Exposure to radiation provokes cellular responses, which are likely regulated by gene expression networks. MicroRNAs are small non-coding RNAs, which regulate gene expression by promoting mRNA degradation or inhibiting protein translation. The expression patterns of both mRNA and miRNA during the radiation-induced lung injury (RILI) remain less characterized and the role of mi -RNAs in the regulation of this process has not been studied. The present study sought to evaluate miRNA and mRNA expression profiles in the rat lung after irradiation.

 

Methods and materials: Male Wistar rats were subjected to single dose irradiation with 20 Gy using 6 MV x-rays to the right lung. (A dose rate of 5Gy/min was applied). Rats were sacrificed at 3, 12 and 26 weeks after irradiation, and morphological changes in the lung were examined by haematoxylin and eosin. The miRNA and mRNA expression profiles were evaluated by microarrays and followed by quant -itative RT-PCR analysis.

 

Results

A cDNA microarray analysis found 2183 transcripts being up-regulated and 2917 transcripts down-regulated (P <= 0.05, >=2.0 fold change) in the lung tissues after irradiation. Likewise, a miRNAs microarray analysis indicated 15 miRNA species being up-regulated and 8 down-regulated (P <= 0.05). Subsequent bioinformatics anal -yses of the differentially expressed mRNA and miRNAs revealed that alterations in mRNA expression following irradiation were negatively correlated with miRNAs expression.

 

Conclusions

Our results provide evidence indicating that irradiation induces alterations of mRNA and miRNA expression in rat lung and that there is a negative correlation of mRNA and miRNA expression levels after irradiation. These findings significantly advance our understanding of the regulatory mechanisms underlying the pathophysiology of radiation-induced lung injury. In summary, RILI does not develop gradually in a linear process. In fact, different cell types interact via cytokines in a very complex network. Furthermore, this study suggests that microRNAs may serve an important role in the pathogenesis of RILI and that understanding their role in RILI may have a significant effect on patient management and diagnosis in the future.

 

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