BioAssist S色谱柱分离IgM的应用
Abstract
A novel macroporous ion exchange material has been developed to provide high retention and capacity of large biomolecules such as IgM. TSKgel BioAssist columns are offered in an analytical format and most recently in a new 13micron particle size for semi-preparative purifications.
Introduction
The use of monoclonal antibodies (MAb) is becoming more important in diagnosis and treatment of disease. IgM in particular has shown to possess unique and beneficial characteristics relative to other immunoglobulin classes; it is a large molecule comprised of five IgG subunits, resulting in a relatively unstable and difficult to purify molecule. Unlike single chain antibodies, IgM cannot be purified by Protein A (an affinity material commonly used for it's high binding capacity and excellent selectivity for antibodies) due to steric hindrance. Alternative affinity methods have been developed with thiophillic absorbents but often result in low binding capacity. In this note we examine an alternative purification method of IgM by ion exchange chromatography using a novel high capacity stationary phase.