SWXL 系列色谱柱对标准蛋白混合样品的分析（2）
色谱条件
色谱柱 : TSKgel SWXL series
流动相 : 20mmol/L Phosphate buffer + 0.3mol/L NaCl (pH7.0)
流速 : 0.5mL/min
检测 : UV (280nm)
温度 : 25C
进样体积 : 10μL
样品 : standard protein (high molecular weight)
液相色谱仪 : Agilent 1120 Compact LC (gradient auto system)
样品组成 :
1. Mouse monoclonal antibody, IgM (M.W.:900KDa, in house, 3g/L)
2. Thyroglobulin (M.W.:667KDa, bovine thyroid, Sigma T1001, 3g/L)
3. Glutamate dehydrogenase (M.W.:280KDa, Wako pure chemicals, Japan, 306-50844, 4g/L)
4. Mouse monoclonal antibody, IgG1 (M.W.:150KDa, in house, 2g/L)
5. Conalbumin (M.W.:47KDa, Wako pure chemicals, Japan, 016-17073, 4g/L)