SuperSW mAb HR色谱柱从单抗半体中分离单体（AN58）
Introduction
Monoclonal antibody (mAb) research continues to grow in an effort to develop effective biotherapeutics for a wide range of diseases. Recent research has shown an interest in mAb half-bodies as therapeutic vectors as they can be further targeted for conjugation, enzyme labeling, or antibody immobilization. Monoclonal antibody half-bodies can be generated through the genetic engineering of cells or by selective reduction of hinge-region disulfide bonds present in the mAb by mild reducing agents, such as TCEP [tris(2-carboxyethyl)phosphine]. Due to its lack of odor and resistance to oxidation in the presence of air, TCEP is a stable reducing agent commonly used in mAb half-body formation.
A mAb half-body was generated through protein reduction using TCEP and subsequently identified by gel electrophoresis for use in this study. The superior resolution obtained between a monoclonal antibody monomer and
half-body species using a TSKgel SuperSW mAb HR column is demonstrated in this application note.
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