不同多组学 LC/MS 系统分析蛋白代谢物和脂质差异

使用 Xevo G2-S QTof 和 TransOmics 多组学 LC/MS 系统分析蛋白代谢物和脂质差异

Abstract：This application highlights a method for the analysis of 26 opioid drugs and metabolites by mixed-mode SPE followed by UPLC®-MS/MS. Glucuronide metabolites are directly analyzed, eliminating the need for enzymatic or chemical hydrolysis.

The method presented here demonstrates the advantages of mixed-mode μElution SPE combined with UPLC-MS/MS for the analysis of 26 opioid compounds and metabolites of interest. All compounds are analyzed in under 5.5 minutes with complete resolution of all isobaric compound pairs. Linearity, analytical accuracy and precision were excellent over the entire calibration range for all 26 compounds. The μElution format enabled the extraction of 100 μL aliquots of oral fluid, leaving the remaining sample for additional assays, or repeat analyses, if necessary. The ability to achieve LOQs of 5 ng/mL for nearly all analytes and the ability to measure glucuronide metabolites directly without hydrolysis make this method well suited for the analysis of these compounds in oral fluid.

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