沉默调节蛋白1(SIRT1) 蛋白 操作指南 英文:Polyclonal Antibody to Sirtuin 1 (SIRT1) Protein [ PRODUCT INFORMATION ] Immunogen: SIRT1 Clonality: Polyclonal (多抗) Host: Rabbit (兔) Species Reactivity: Mouse (小鼠) Ig type: Rabbit IgG (兔IgG) Purification: Antibodies are purified by target protein affinity chromatography. Applications: WB, ICC, IHC-P, IHC-F Form: Liquid Size: 100μg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant mouse SIRT1 (Glu236~Ala490) expressed in E.coli. Molecular Weight: 30.3 kDa USCN accession No.: P94912Mu01 Sequence: The target protein is fused with a His-tag and its sequence is listed below. [ ANTIBODY SPEFICITY ] Anti SIRT1 is a rabbit polyclonal antibody raised against mouse SIRT1. This antibody has been selected for its ability to recognize mouse SIRT1 in immunohistochemical staining and western blotting, non cross-reactive with other members of the family. [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH 7.4, containing 0.02%Na3N, 50% glycerol. [ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. |