The Asparaginase Activity Assay Kit provides a simple and direct procedure for measuring Asparaginse activity in a variety of biological samples. Asparaginase activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex= 535/λem= 587 nm) product, proportional to the aspartate generated. One milliunit (mU) of Asparaginase is defined as the amount of enzyme that catalyzes the formation of 1.0 mmole of aspartate per minute at 25 °C.