The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
The clone 2D7/1 has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium.
Tissue, cells or virus corresponding to Escherichia coli E. coli LPS. Escherichia coli J5 cells.
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