AssayMax Human Elastase (ELA-2) ELISA Kit 中性粒细胞弹性蛋白酶ELA-2酶联免疫试剂盒
Introduction Human Neutrophil Elastase (NE), also known as ELA2, is a subfamily of serine proteinase elastases. It consists of 218 amino acid residues with a molecular weight of 23 kDa and two asparagine-linked carbohydrate side chains (1). NE is stored in neutrophil lysosomes azurophil granules during neutrophil differentiation and is involved in a variety of immune defense reactions and degenerative and inflammatory diseases (2-3). Upon infection, the activated neutrophils release NE which then hydrolyzes azurophil granule proteins and extracellular matrix proteins: elastin, collagen, and proteoglycan (4). As a host defense protein, NE degrades bacterial outer membranes and virulence proteins (5-6). When expressed abnormally, NE causes the development of pulmonary emphysema (7). NE mutations are associated with cyclic neutropenia and severe congenital neutropenia (8-9).
Principle of the Assay The AssayMax Human Neutrophil Elastase ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human neutrophil elastase in plasma, serum, urine, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human elastase in less than 4 hours. A polyclonal antibody specific for human elastase has been pre-coated onto a 96-well microplate with removable strips. Elastase in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for elastase, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.