AssayMax Human Complement C3 ELISA Kit 补体C3酶联免疫试剂盒
Introduction Complement component 3 (C3) plays a central role in all three complement activation pathways. The C3 precursor contains 1663 amino acids and has a molecular weight of about 180 kDa (1). Human C3 has 77% identity to mouse C3 at the amino acid level (2). C3 is cleaved by C3 convertase into two activated fragments C3a and C3b. The anaphylatoxin C3a is a vasoactive peptide and a mediator of local inflammatory process (3). The C3b in complex with receptor can bind covalently to pathogen surfaces to promote phagocytosis (4 - 5). Acquired C3 deficiency is associated with severe recurrent meningococcal and pneumococcal infections (6). Plasma C3 and C3a levels are elevated in cryptogenic and large-vessel disease subtypes of ischemic stroke (7).
Principle of the Assay The AssayMax Human Complement C3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human complement C3 in plasma and serum samples. This assay employs a quantitative competitive enzyme immunoassay technique that measures human complement C3 in less than 3 hours. A polyclonal antibody specific for human complement C3 has been pre-coated onto a 96-well microplate with removable strips. Complement C3 in standards and samples is competed with a biotinylated complement C3 sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.